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Neuronal endoplasmic reticulum acts as a single functional Ca2+ store shared by ryanodine and inositol-1,4,5-trisphosphate receptors as revealed by intra-ER [Ca2+] recordings in single rat sensory neurones

  • Cellular Neurophysiology
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Abstract

We addressed the fundamentally important question of functional continuity of endoplasmic reticulum (ER) Ca2+ store in nerve cells. In cultured rat dorsal root ganglion neurones we measured dynamic changes in free Ca2+ concentration within the ER lumen ([Ca2+]L) in response to activation of inositol-1,4,5-trisphosphate receptors (InsP3Rs) and ryanodine receptors (RyRs). We found that both receptors co-exist in these neurones and their activation results in Ca2+ release from the ER as judged by a decrease in [Ca2+]L. Depletion of Ca2+ stores following an inhibition of sarco(endoplasmic)reticulum Ca2+-ATPase by thapsigargin or cyclopiazonic acid completely eliminated Ca2+ release via both InsP3Rs and RyRs. Similarly, when the store was depleted by continuous activation of InsP3Rs, activation of RyRs (by caffeine or 0.5 μM ryanodine) failed to produce Ca2+ release, and vice versa, when the stores were depleted by activators of RyRs, the InsP3-induced Ca2+ release disappeared. We conclude that in mammalian neurones InsP3Rs and RyRs share the common continuous Ca2+ pool associated with ER.

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Acknowledgements

This research was supported by a BBSRC research grant to A.V. (ref. 34/C12751). The authors are grateful to Prof. D. Tomlinson for helpful comments on the manuscript.

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  1. The University of Manchester, School of Biological Sciences, 1.124 Stopford Building, Oxford Road, Manchester , M13 9PT, UK

    Natasha Solovyova & Alexei Verkhratsky

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  1. Natasha Solovyova
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  2. Alexei Verkhratsky
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Correspondence to Alexei Verkhratsky.

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Solovyova, N., Verkhratsky, A. Neuronal endoplasmic reticulum acts as a single functional Ca2+ store shared by ryanodine and inositol-1,4,5-trisphosphate receptors as revealed by intra-ER [Ca2+] recordings in single rat sensory neurones. Pflugers Arch - Eur J Physiol 446, 447–454 (2003). https://doi.org/10.1007/s00424-003-1094-z

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  • DOI: https://doi.org/10.1007/s00424-003-1094-z

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