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Secretoglobins in the human pituitary: high expression of lipophilin B and its down-regulation in pituitary adenomas

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Abstract

Secretoglobins are small secreted proteins, the expression of which has mostly been associated with secretory mucosal epithelia. Several secretoglobins have been implicated in the development of various human cancers. Allelic deletions of chromosome 11q13 correlates with the invasiveness of pituitary tumors. Intriguingly, several secretoglobin genes are located on 11q13; however, for most of these genes the expression in the pituitary and pituitary tumors have not been investigated. Antibodies specific for the secretoglobin lipophilin B (SCGB1D2, BU101) were developed and used in an immunohistochemical analysis of a human normal tissue microarray. Prominent lipophilin B immunoreactivity was found in the secretory cells of the anterior pituitary. Eight of nine analyzed pituitary adenomas showed a reduction in lipophilin B immunoreactivity compared to normal pituitary. However, there was no apparent association between lipophilin B immunoreactivity and hormone production or tumor invasiveness. Expression of eight different secretoglobin mRNAs were analyzed in normal pituitary and the pituitary adenoma cell line HP75 by highly specific quantitative real-time reverse transcription-PCR assays. Lipophilins B and C (SCGB2A1, mammaglobin B) were the most prominently expressed secretoglobin mRNAs in the pituitary. No secretoglobin mRNA was detected in the HP75 cells. The present report demonstrates, for the first time, lipophilin B expression in the pituitary and its apparent down-regulation in pituitary adenomas.

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Fig. 1 A, B
Fig. 2 A–F

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Acknowledgements

This work was supported by grants from the Cancer Research Foundation in Northern Sweden and the Swedish Cancer Society. We thank Kerstin Bergh for help with the immunohistochemistry, and Mikael Kimdal for help with the recombinant proteins.

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Authors and Affiliations

  1. Department of Radiation Sciences, Oncology, Umeå University, 901 87, Umeå, Sweden

    Anna Sjödin, Dongsheng Guo, Roger Henriksson & Håkan Hedman

  2. Department of Neurosurgery, Institute of Surgical Science, Haukeland University Hospital, Bergen, Norway

    Morten Lund-Johansen

  3. Department of Pathology, The Gade Institute, Haukeland University Hospital, Bergen, Norway

    Bård Kronen Krossnes & Peer Lilleng

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  1. Anna Sjödin
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  2. Dongsheng Guo
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  3. Morten Lund-Johansen
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  4. Bård Kronen Krossnes
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  5. Peer Lilleng
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  6. Roger Henriksson
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  7. Håkan Hedman
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Correspondence to Håkan Hedman.

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Sjödin, A., Guo, D., Lund-Johansen, M. et al. Secretoglobins in the human pituitary: high expression of lipophilin B and its down-regulation in pituitary adenomas. Acta Neuropathol 109, 381–386 (2005). https://doi.org/10.1007/s00401-004-0972-6

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  • DOI: https://doi.org/10.1007/s00401-004-0972-6

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