Skip to main content
SpringerLink
Log in

High-resolution mapping of a linkage group on mouse chromosome 8 conserved on human chromosome 16Q

  • Original Contribution
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

We have performed a high-resolution linkage analysis for the conserved segment on distal mouse Chromosome (Chr) 8 that is homologous to human Chr 16q. The interspecific backcross used involved M. m. molossinus and an M. m. domesticus line congenic for an M. spretus segment from Chr 8 flanked by phe-notypic markers Os (oligosyndactyly) and e, a coat colormarker. From a total of 682 N2 progeny, the 191 animals revealing a recombination event between these phenotypic markers were typed for 23 internal loci. The following locus order with distances in cM was obtained: (centromere)-Os-4.1-Mmp2-0.2-Ces1,Es1, Es22-1.2-Mt1,D8Mitl5-2.2-Got2, D8Mit11-3.7-Es30-0.3-Es2, Es7-0.9-Ctral,Lcat-0.3-Cdh1, Cadp, Nmor1, D8Mit12-0.2-Mov34-2.5-Hp, Tat-0.2-Zfp4-1.6-Zfpl,Ctrb-10.9-e. In a separate interspecific cross involving 62 meioses, Dpep1 was mapped together with Aprt and Cdh3 at 12.9 cM distal to Hp, Tat, to the vicinity of e. Our data give locus order for markers not previously resolved, add Mmp2 and Dpepl as new markers on mouse Chr 8, and indicate that Ctral is the mouse homolog for human CTRL. Comparison of the order of 17 mouse loci with that of their human homologs reveals that locus order is well conserved and that the conserved segment in the human apparently spans the whole long arm of Chr 16.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abonia JP, Abel KJ, Eddy RL, Elliott RW, Chapman VM, Shows TB, Gross KW (1993) Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new linkage conservation. Mamm Genome 4, 25–32

    Article  PubMed  CAS  Google Scholar 

  • Adachi H, Tawaragi Y, Inuzuka C, Kubota I, Tsujimoto M, Nishihara T, Nakazato H (1990) Primary structure of human microsomal dipeptidase deduced from molecular cloning. J Biol Chem 265, 3992–3995

    PubMed  CAS  Google Scholar 

  • Alonso S, Montagutelli X, Simon-Chanzottes D, Guénet J-L, Buckingham M (1993) Re-localization of Actsk-1 to mouse chromosome 8, a new region of homology with human chromosome 1. Mamm Genome 4, 15–20

    Article  PubMed  CAS  Google Scholar 

  • Andrews SJ (plus 17 co-authors) (1981) Mouse Newslett 65, 18–22

    Google Scholar 

  • Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD (1995) Phospholipase C gamma-2 (Pclg2) and phos-pholipase C gamma-1 (Pclg1) map to distinct regions in the human and mouse genomes. Genomics 25, 29–35

    Article  PubMed  CAS  Google Scholar 

  • Ashworth A, Williams BP, Buchberg AM, Goodfellow PN, Solomon E, Potter J, Willison KR (1989) Chromosomal localization of zinc finger protein genes in man and mouse. Genomics 4, 323–327

    Article  PubMed  CAS  Google Scholar 

  • Ballabio A (1993) The rise and fall of positional cloning? Nature Genet 3, 277–279

    Article  PubMed  CAS  Google Scholar 

  • Becker-Follmann J, Kaupmann K, Vestweber D, Jockusch H, Starzinski-Powitz A, Scherer G (1992) Assignment of muscle cadherin (MCAD) to human chromosome 16q24-qter and to mouse chromosome 8, and of placental cadherin (PCAD)-like sequences PCADL1 to 16q22.1 and PCADL2 to 16q24-qter. Cytogenet Cell Genet 60, 168

    Google Scholar 

  • Bell GI, Quinto C, Quiroga M, Valenzuela P, Craik CS, Rutter WJ (1984) Isolation and sequence of a rat chymotrypsinogen B gene. J Biol Chem 259, 14265–14270

    PubMed  CAS  Google Scholar 

  • Callen DF, Lane SA, Kozman H, Kremmidiotis G, Whitmore SA, Lowenstein M, Doggett NA, Kenmochi N, Page DC, Maglott DR, Nierman WC, Murakawa K, Berry R, Sikela JM, Houlgatte R, Auffray C, Sutherland GR (1995) Integration of transcript and genetic maps of chromosome 16 at near-1-Mb resolution: demonstration of a “hot spot” for recombination at 16pl2. Genomics 29, 503–511

    Article  PubMed  CAS  Google Scholar 

  • Casamassima AC, Klein RM, Wilmot PL, Brenholz P, Lawrence RS (1990) Deletion of 16q with prolonged survival and unusual radio-graphic manifestations. Am J Med Genet 37, 504–509

    Article  PubMed  CAS  Google Scholar 

  • Castilla EE, Paz JE, Orioli-Parreiras IM (1980) Syndactyly: frequency of specific types. Am J Med Genet 5, 357–364

    Article  PubMed  CAS  Google Scholar 

  • Ceci JD (1994) Mouse Chromosome 8. Mamm Genome 5 (Suppl), S124-S138

    PubMed  CAS  Google Scholar 

  • Ceci JD, Justice MJ, Lock LF, Jenkins NA, Copeland NG (1990) An interspecific backcross linkage map of mouse chromosome 8. Genomics 6, 72–79

    Article  PubMed  CAS  Google Scholar 

  • Chowdury K, Dietrich S, Balling R, Guénet J-L, Gruss P (1989) Structure, expression and chromosomal location of Zfp-1, a murine zinc finger protein gene. Nucleic Acids Res 17, 10427–10438

    Article  Google Scholar 

  • Dib C, Fauré S, Fitames C, Samson D, Drouot N, Vignal A, Millasseau P, Marc S, Hazan J, Seboun E, Lathrop M, Gyapay G, Morissette J, Weis-senbach J (1996) A comprehensive genetic map of the human genome based on 5,264 microsatellite markers. Nature 380, 152–154

    Article  PubMed  CAS  Google Scholar 

  • Dietrich W, Katz H, Lincoln S, Shin H-S, Friedman J, Dracopoli NC, Lander E (1992) A genetic map of the mouse suitable for typing intra-specific crosses. Genetics 131, 423–447

    PubMed  CAS  Google Scholar 

  • Dietrich WF, Miller J, Steen R, Merchant MA, Damron-Boles D, Husain Z, Dredge R, Daly MJ, Ingalls KA, O’Connor TJ, Evans CA, DeAngelis MM, Levinson DM, Kruglyak L, Goodman N, Copeland NG, Jenkins NA, Hawkins TL, Stein L, Page DC, Lander ES (1996) A comprehensive genetic map of the mouse genome. Nature 380, 149–152

    Article  PubMed  CAS  Google Scholar 

  • Dogget NA, Callen DF (1995) Report of the third international workshop on human chromosome 16 mapping 1994. Cytogenet Cell Genet 68, 165–184

    Article  Google Scholar 

  • Donalies M, Cramer M, Ringwald M, Starzinski-Powitz A (1991) Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells. Proc Natl Acad Sci USA 88, 8024–8028

    Article  PubMed  CAS  Google Scholar 

  • Eistetter HR, Adolph S, Ringwald M, Simon-Chazottes D, Schuch R, Guénet J-L, Kemler R (1988) Chromosomal mapping of the structural gene coding for the mouse cell adhesion molecule uvomorulin. Proc Natl Acad Sci USA 85, 3489–3493

    Article  PubMed  CAS  Google Scholar 

  • Elder FF, Ferguson JW, Lockhart LH (1984) Identical twins with deletion 16q syndrome: evidence that 16ql2.2–ql3 is the critical band region. Hum Genet 67, 233–236

    Article  PubMed  CAS  Google Scholar 

  • Genetta TL, D’Eustachio P, Kadner SS, Finley TH (1988) cDNA cloning of esterase 1, the major esterase activity in mouse plasma. Biochem Biophys Res Commun 151, 1364–1370

    Article  PubMed  CAS  Google Scholar 

  • Gridley T, Gray DA, Orr-Weaver T, Soriano P, Barton DE, Francke U, Jänisch R (1990) Molecular analysis of the Mov-34 mutation: transcript disrupted by proviral integration in mice is conserved in Drosophila. Development 109, 235–242

    PubMed  CAS  Google Scholar 

  • Gridley T, Jänisch R, Gendron-Maguire M (1991) The murine Mov-34 gene: full-length cDNA and genomic organization. Genomics 11, 501–507

    Article  PubMed  CAS  Google Scholar 

  • Grüneberg H (1956) Genetical studies on the skeleton of the mouse. XVIII. Three genes for syndactylism. J Genet 54, 113–145

    Article  Google Scholar 

  • Howard TA, Rochelle JM, Saunders AM, Seldin MF (1991) A linkage map of mouse chromosome 8: further definition of homologous linkage reltaionships between mouse chromosome 8 and human chromosome 8,16, and 19. Genomics 10, 207–213

    Article  PubMed  CAS  Google Scholar 

  • Huhtala P, Eddy RL, Fan YS, Byers MG, Shows TB, Tryggvason K (1990) Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16. Genomics 6, 554–559

    Article  PubMed  CAS  Google Scholar 

  • Kaupmann K, Becker-Follmann J, Scherer G, Jockusch H, Starzinski-Powitz A (1992) The gene for the cell adhesion molecule M-cadherin maps to mouse chromosome 8 and human chromosome 16q24.1-qter and is near the E-cadherin (uvomorulin) locus in both species. Genomics 14, 488–490

    Article  PubMed  CAS  Google Scholar 

  • Larsen F, Solheim J, Kristensen T, Kolsto A-B, Prydz H (1993) A tight cluster of five unrelated human genes on chromosome 16q22.1. Hum Mol Genet 2, 1589–1595

    Article  PubMed  CAS  Google Scholar 

  • Müller G, Scherer G, Zentgraf H, Ruppert S, Herrmann B, Lehrach H, Schütz G (1985) Isolation, characterization and chromosomal mapping of the mouse tyrosine aminotransferase gene. J Mol Biol 184, 367–373

    Article  PubMed  Google Scholar 

  • Nakagawa H, Inazawa J, Inoue K, Misawa S, Kashima K, Adachi H, Nakazato H, Abe T (1991) Assignment of the human renal dipeptidase gene (DPEP1) to band q24 of chromosome 16. Cytogenet Cell Genet 58, 2002

    Google Scholar 

  • Obaru K, Nomiyama H, Shimada K, Nagashima F, Morino Y (1986) Cloning and sequence analysis of mRNA for mouse aspartate aminotransferase isoenzymes. J Biol Chem 261, 16976–16983

    PubMed  CAS  Google Scholar 

  • O’Brien SJ, Marshall Graves JA (1991) Report of the committee on comparative gene mapping. Eleventh international workshop on human gene mapping. Cytogenet Cell Genet 50, 1129–1151

    Google Scholar 

  • O’Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG (1993) Anchored reference loci for comparative genome mapping in mammals. Nature Genet 3, 103–112

    Article  PubMed  CAS  Google Scholar 

  • Ovnic M, Tepperman K, Medda S, Elliott RW, Stephenson DA, Grant SG, Ganschow RE (1991) Characterization of a murine cDNA encoding a member of the carboxylesterase multigene family. Genomics 9, 344–354

    Article  PubMed  CAS  Google Scholar 

  • Reeves RH, Crowley MR, O’Hara BF, Gearhart JD (1990) Sex, strain, and species differences affect recombination across an evolutionarily conserved segment on mouse chromosome 16. Genomics 8, 141–148

    Article  PubMed  CAS  Google Scholar 

  • Robbi M, Beaufay H, Octave J-N (1990) Nucleotide sequence of cDNA coding for rat liver pI 6.1 esterase (ES-10), a carboxylesterase located in the lumen of the endoplasmic reticulum. Biochem J 269, 451–458

    PubMed  CAS  Google Scholar 

  • Ronai A, Berning W, Gaa A, von Deimling O (1993) Esterase-30 (ES-30) of the house mouse: biochemical characterization and genetics of a new carboxylesterase isozyme linked to cluster-2 loci on chromosome 8. Biochem Genet 31, 279–294

    Article  PubMed  CAS  Google Scholar 

  • Ruppert S, Kelsey G, Schedl A, Schmid E, Thies E, Schütz G (1992) Deficiency of an enzyme of tyrosine metabolism underlies altered gene expression in newborn liver of lethal albino mice. Genes Dev 6, 1430–1443

    Article  PubMed  CAS  Google Scholar 

  • Schaller E, von Deimling O (1979) Methionine-α-naphthyl ester, a useful chromogenic substrate for esterproteases of the mouse submandibular gland. Anal Biochem 93, 251

    Article  PubMed  CAS  Google Scholar 

  • Scherer G, Bausch E, Gaa A, von Deimling O (1989) Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5, 275–282

    Article  PubMed  CAS  Google Scholar 

  • Searle AG (1968) An extension series in the mouse. J Hered 59, 341–342

    PubMed  CAS  Google Scholar 

  • Searle PF, Davison BL, Stuart GW, Wilkie TM, Norstedt G, Palmiter RD (1984) Regulation, linkage, and sequence of mouse metallothionein I and II genes. Mol Cell Biol 4, 1221–1230

    PubMed  CAS  Google Scholar 

  • Shapiro M, Kozak CA (1991) Genetic mapping in the mouse of four loci related to the jun family of transcriptional activators. Somatic Cell Mol Genet 17, 341–347

    Article  CAS  Google Scholar 

  • Tata F, Chaves ME, Markham AF, Scrace GD, Waterfield MD, McIntyre N, Williamson R, Humphries SE (1987) The isolation and characterization of cDNA and genomic clones for human lecithin: cholesterol acyl-transferase. Biochim Biophys Acta 910, 142–148

    PubMed  CAS  Google Scholar 

  • Tsuzuki T, Obaru K, Setoyama C, Shimada K (1987) Structural organization of the mouse mitochondrial aspartate aminotransferase. J Mol Biol 198, 21–31

    Article  PubMed  CAS  Google Scholar 

  • von Deimling O, Wienker TF (1992) Chromosome 8 controlled esterases of the house mouse. Adv Electrophor 5, 285–350

    Google Scholar 

  • von Deimling OH, Gröning J, Gaa A (1991) Genetic and biochemical characterization of a new chymotrypsin isozyme of the house mouse, CTRA-1. Biochem Genet 29, 545–557

    Article  Google Scholar 

  • Watanabe T, Shimizu A, Ohno K, Masaki S, Kondo K (1989) Restriction fragment length variations and chromosome mapping of two mouse metallothionein genes, Mt-1 and Mt-2. Biochem Genet 27, 689–697

    PubMed  CAS  Google Scholar 

  • Womack JE (1975) Esterase-6 (Es-6) in laboratory mice: hormone-influenced expression and linkage relationship to oligosyndactylism (Os), esterase-1 (Es-1), and esterase-2 (Es-2) in chromosome 8. Biochem Genet 13, 311–322

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Johannes Becker-Follmann

    Present address: Suntory, Institute for Biomedical Research, 1-1-1 Wakayamadai, 618, Shimamoto-cho, Mishima-gun, Osaka, Japan

Authors and Affiliations

  1. Institute of Human Genetics, University of Freiburg, Breisacherstr. 33, D-79106, Freiburg, Germany

    Johannes Becker-Follmann, Elke Baùsch, Ernst Natt & Gerd Scherer

  2. Institute of Pathology, Department of Chemical Pathology, University of Freiburg, Albertstr. 19, D-79104, Freiburg, Germany

    Andreas Gaa & Otto von Deimling

Authors
  1. Johannes Becker-Follmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andreas Gaa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elke Baùsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ernst Natt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gerd Scherer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Otto von Deimling
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Becker-Follmann, J., Gaa, A., Baùsch, E. et al. High-resolution mapping of a linkage group on mouse chromosome 8 conserved on human chromosome 16Q. Mammalian Genome 8, 172–177 (1997). https://doi.org/10.1007/s003359900384

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s003359900384

Keywords

Search

Navigation