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Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays

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Abstract.

Objective: Differences between detection techniques may be partly responsible for variable mediator concentrations reported in exhaled breath condensate (EBC). We compared two types of immunoassays to estimate thromboxane A2 (TxA2) concentration.

Materials and methods: Thromboxane B2 (TxB2) levels were measured by enzyme immunoassay (EIA) and TxB2/2,3-dinor TxB2 by radioimmunoassay (RIA) in 10 healthy subjects and 13 asthmatic patients. 2,3-Dinor TxB2 was also determined by a separate EIA.

Results: Thromboxane was detected in all samples by RIA, but only in about 75% of samples by EIA. 2,3-Dinor TxB2 was detected in most samples. There was no agreement between the results of the different immunoassays. As compared to healthy subjects, exhaled breath condensates of asthmatic patients contained significantly more immunoreactivity by RIA and TxB2 EIA (but not by 2,3-dinor TxB2 EIA).

Conclusion: RIA and EIA resulted in vastly different absolute values. The difference found between healthy volunteers and asthmatic patients however, suggests an increased level of TxA2 in the airways of asthmatics.

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Abbreviations

BAL:

bronchoalveolar lavage

COX:

cyclo-oxygenase

cysLTs:

cysteinyl leukotrienes

EBC:

exhaled breath condensate

EIA:

enzyme immunoassay

FENO:

exhaled nitric oxide

FEV1:

forced expiratory volume in 1 second

LTB4:

leukotriene B4

LTE4:

leukotriene E4

RIA:

radioimmunoassay

TxA2:

thromboxane A2

TxB2:

thromboxane B2

2,3-dinor TxB2:

2,3-dinor thromboxane B2

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Authors and Affiliations

  1. Departments of Pathophysiology, National Korányi Institute for TB and Pulmonology, Budapest, Hungary

    É. Huszár, Á. Jakab, I. Barta & I. Horváth

  2. Departments of Bronchology, National Korányi Institute for TB and Pulmonology, Budapest, Hungary

    Z. Szabó

  3. Departments of Pulmonology, National Korányi Institute for TB and Pulmonology, Budapest, Hungary

    I. Herjavecz

Authors
  1. É. Huszár
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  2. Z. Szabó
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  3. Á. Jakab
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  4. I. Barta
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  5. I. Herjavecz
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  6. I. Horváth
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Corresponding author

Correspondence to I. Horváth.

Additional information

Received 25 August 2004; returned for revision 12 November 2004; accepted by M. J. Parnham 25 May 2005

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Huszár, É., Szabó, Z., Jakab, Á. et al. Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays. Inflamm. res. 54, 350–355 (2005). https://doi.org/10.1007/s00011-005-1361-x

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  • DOI: https://doi.org/10.1007/s00011-005-1361-x

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