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Induction of cyclooxygenase protein and stimulation of prostaglandin E2 release by epidermal growth factor in cultured guinea pig gastric mucous cells

  • Esophageal, Gastric, and Duodenal Disorders
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Abstract

The present study was undertaken to investigate whether epidermal growth factor (EGF) could stimulate prostaglandin E2 release, and if so, by what mechanism EGF would exert such an effect in gastric mucosal cells. In cultured guinea pig gastric mucous cells, EGF dosedependently stimulated prostaglandin E2 release, with maximal stimulation observed at 10 ng/ml. EGF stimulated an increase in cyclooxygenase activity, which was reduced by protein synthesis inhibitor, actinomycin D, and cycloheximide. EGF also stimulated the enzyme protein synthesis estimated by Western blot analysis, whereas EGF did not stimulate phospholipase A2 activity. These results suggest that such an effect of EGF onde novo synthesis of cyclooxygenase protein and prostaglandin E2 release may be involved at least in part in the mechanism of EGF-induced local regulation of gastric mucosal integrity.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, 650, Kobe, Japan

    Osamu Nakano MD, PhD,  Choitsu Sakamoto MD, PhD, Kohei Matsuda MD, PhD, Yoshitaka Konda MD, PhD, Takashi Matozaki MD, PhD, Hogara Nishisaki MD, PhD, Ken Wada MD, PhD, Toshiya Suzuki MD, Toru Uchida MD, Munehiko Nagao MD, PhD & Masato Kasuga MD, PhD

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  1. Osamu Nakano MD, PhD
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  2. Choitsu Sakamoto MD, PhD
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  3. Kohei Matsuda MD, PhD
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  4. Yoshitaka Konda MD, PhD
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  5. Takashi Matozaki MD, PhD
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  6. Hogara Nishisaki MD, PhD
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  7. Ken Wada MD, PhD
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  8. Toshiya Suzuki MD
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  9. Toru Uchida MD
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  10. Munehiko Nagao MD, PhD
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  11. Masato Kasuga MD, PhD
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Nakano, O., Sakamoto, C., Matsuda, K. et al. Induction of cyclooxygenase protein and stimulation of prostaglandin E2 release by epidermal growth factor in cultured guinea pig gastric mucous cells. Digest Dis Sci 40, 1679–1686 (1995). https://doi.org/10.1007/BF02212688

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  • DOI: https://doi.org/10.1007/BF02212688

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