Abstract
Recently, there has been an increasing interest in the involvement of adenosine as a mediator of allergic asthma. In the present study, we have employed an allergic rabbit model to study the airway responses to adenosine. The rabbit litter mates were injected i.p., within 24 h of birth, with ragweed pollen extract to preferentially produce ragweed-specific IgE. This allergic rabbit model demonstrates many clinical features of asthma similar to human allergic asthma. Aerosolized adenosine (0.156–10 mg/ml) caused a concentration-dependent bronchoconstriction. The normal rabbits (non-immunized) did not respond to adenosine within that concentration range. Theophylline significantly inhibited the adenosine-induced bronchoconstriction. These early studies suggest that adenosine differentiated asthmatic airways from normal airways and adenosine-induced bronchoconstriction in this model was mediated through specific cell surface receptor(s).
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References
M. J. Cushley, A. E. Tattersfield and S. T. Holgate,Inhaled adenosine and guanosine on airway resistance in normal and asthmatic subjects. Br. J. Clin. Pharmacol.15, 161–165 (1983).
M. J. Cushley, A. E. Tattersfield and S. T. Holgate,Adenosine induced bronchoconstriction in asthma: antagonism by inhaled theophylline. Am. Rev. Respir. Dis.129, 380–384 (1984).
J. S. Mann, S. T. Holgate, A. G. Renwick and M. J. Cushley,Airway effects of purine nucleosides and nucleotides and release with provocation in asthma. J. Appl. Physiol61, 1667–1667 (1986).
B. B. Fredhom,The release of adenosine from rat lung by antigen and compound 48/80. Acta Physiol. Scand.111, 507–508 (1981).
T. Bjorck, Y. Harada, E. Wikstrom, L. E. Gustafsson, P. Hedquist, B. Dahlen, D. Zetterstrom, G. Unge and S. E. Dahlen,Comparison of bronchial reactivity in vitro between asthmatic and nonasthmatics. Am. Rev. Respir. Dis.143, A409 (1991).
W. J. Metzger, Late phase asthma in an allergic rabbit model. InLate Phase Allergic Reactions (Ed. W. Dorsch) pp. 347–362. CRC Press, Florida 1990.
A. J. Coyle, C. P. Page, L. Atkinson, L. Sjoerdsma, D. Touvay and W. J. Metzger,Modification of late onset airway obstruction and bronchial hyperresponsiveness in an allergic rabbit model by selective platelet activating factor antagonists BN 52021. J. Allergy Clin. Immunol.84 960–967 (1989).
R. E. Giles, M. P. Finkel and J. Mazurowski,Use of an analog on-line computer for the evaluation of pulmonary resistance and dynamic compliance in the anesthetized dog. Arch. Int. Pharmacodyn. Ther.194, 213–222 (1971).
A. G. Driver, C. A. Kukoly, W. J. Metzger and S. J. Mustafa,Bronchial challenge with adenosine causes the release of serum neutrophil chemotactic factor in asthma. Am. Rev. Respir. Dis.143, 1002–1007 (1991).
S. T. Holgate, J. P. Fennerty and R. Polosa,Mechanisms of purine-induced bronchoconstriction in asthma. Arch. Int. Pharmacodyn.303, 122–131 (1990).
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Supported in part by NIH grant RO1 AI 26726 and HL 34741 and Ciba-Geigy Corporation.
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Ali, S., Mustafa, S.J. & Metzger, W.J. Adenosine-induced bronchoconstriction in an allergic rabbit model: Antagonism by theophylline aerosol. Agents and Actions 37, 165–167 (1992). https://doi.org/10.1007/BF02028098
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DOI: https://doi.org/10.1007/BF02028098