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On the involvement of a glucose 6-phosphate transport system in the function of microsomal glucose 6-phosphatase

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Summary

A model for microsomal glucose 6-phosphatase (EC 3.1.3.9) is presented. Glucose 6-phosphatase is postulated to be resultant of the coupling of two components of the microsomal membrane: 1) a glucose 6-phosphate — specific transport system which functions to shuttle the sugar phosphate from the cytoplasm to the lumen of the endoplasmic reticulum; and 2) a catalytic component, glucose-6-P phosphohydrolase, bound to the luminal surface of the membrane. A large body of existing data was shown to be consistent with this hypothesis. In particular, the model reconciles well-documented differences in the kinetic properties of the enzyme of untreated and modified microsomal preparations. Characteristic responses of the enzyme to changes in nutritional and hormonal states may be attributed to adaptations which alter the relative capacities of the transport and catalytic components.

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This work was supported in part by Research Grants FR-05462 and AM-15139 from the National Institutes of Health, United States Public Health Service.

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Arion, W.J., Wallin, B.K., Lange, A.J. et al. On the involvement of a glucose 6-phosphate transport system in the function of microsomal glucose 6-phosphatase. Mol Cell Biochem 6, 75–83 (1975). https://doi.org/10.1007/BF01732001

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