Abstract
Radioimmunoassayable gastric inhibitory polypeptide was measured in extracts of canine antrum, duodenum, jejunum, and ileum. The highest GIP concentrations were found in the duodenum (347±53 ng/g) and jejunum (300±68 ng/g). An immunochemical similarity was demonstrable between porcine GIP and canine GIP. Dogs prepared with Mann-Bollman fistulae were given an amino acid (AA) mixture or medium-chain triglycerides (MTC) by intraduodenal perfusion. With AA, a peak mean serum concentration of 672±106 pg/ml was reached 15 min after starting the perfusion. MCT resulted in a peak mean serum GIP concentration of 504±55 pg/ml 30 min after beginning the perfusion. When compared to results previously reported from this laboratory, AA and MCT are not as potent as corn oil (long-chain triglyceride) or glucose in stimulating GIP release. We conclude: (1) Immunoassayable GIP concentrations are highest in the canine proximal small intestine. (2) AA and MCT are weak stimulants of GIP release in the dog.
Similar content being viewed by others
References
Brown JC, Mutt V, Pederson RA: Further purification of a polypeptide demonstrating enterogastrone activity. J Physiol 209:57–64, 1970
Dupré J, Ross SA, Watson D, Brown JC: Stimulation of insulin secretion by gastric inhibitory polypeptide in man. J Clin Endocrinol Metab 37:826–828, 1973
Cataland S, Crockett SE, Brown JC, Mazzaferri EL: Gastric inhibitory polypeptide (GIP) stimulation by oral glucose in man. J Clin Endocrinol Metab 39:223–228, 1974
Falko JM, Crockett SE, Cataland S, Mazzaferri EL: Gastric inhibitory polypeptide (GIP) stimulated by fat ingestion in man. J Clin Endocrinol Metab 41:236–241, 1975
Thomas FB, Cataland S, Crockett SE, Mekhjian SH, and Mazzaferri EL: Stimulation of gastric inhibitory polypeptide and insulin by intraduodenal amino acids. Gastroenterology 70:523–527, 1976
Sirinek KR, Crockett SE, Mazzaferri EL, Cataland S, and Thomford NR: Release of gastric inhibitory polypeptide comparison of glucose and fat as stimuli. Surg Forum 25:361–363, 1974
Kuzio M, Dryburgh JR, Malloy KM, Brown JC: Radioimmunoassay for gastric inhibitory polypeptide. Gastroenterology 66:357–364, 1974
Martin EW, Jr, Sirinek KR, Crockett SE, O'Dorisio TM, Mazzaferri EL, Thomford NR, Cataland S: The release of gastric inhibitory polypeptide: A comparison of hyperosmolar carbohydrate solutions as stimuli. Surg Forum, 26:381–382, 1975
Desbuquois B, Aurbach GD: Use of polyethylene glycol to separate free and antibody bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab 33:732–738, 1971
Brown JC, Dryburgh JR: A gastric inhibitory polypeptide II: The complete amino acid sequence. Can J Biochem 49:867–872, 1971
Polak JM, Bloom SR, Kuzio M, Brown JC, and Pearse AGE: Cellular localization of gastric inhibitory polypeptide in the duodenum and jejunum. Gut 14:284–288, 1973
Bloom SR: Hormones of the gastrointestinal tract. Br Med Bull 30:62–67, 1974
Brown JC: “Enterogastrone” and other new gut peptides. Med Clin N Am 58:1347–1374, 1974
Cataland S, Brooks R, O'Dorisio T, Mekhjian HS: The role of gastric inhibitory polypeptide in duodenal uncer. Gastroenterology 68:14-A, 1975 (abstract)
Greenberger NJ, Skillman TG: Medium chain triglycerides. N Engl J Med 280:1045–1058, 1969
Author information
Authors and Affiliations
Additional information
This study was supported in part by the Bremer Foundation, 521532-7308, the National Institute of Arthritis and Metabolic Disease 5T01-AM05118-19, and General Clinical Research Center Program, National Institutes of Health RR-34, and National Cancer Institute 5F22-CA00776-02.
Rights and permissions
About this article
Cite this article
O'Dorisio, T.M., Cataland, S., Stevenson, M. et al. Gastric inhibitory polypeptide (GIP). Digest Dis Sci 21, 761–765 (1976). https://doi.org/10.1007/BF01073027
Issue Date:
DOI: https://doi.org/10.1007/BF01073027