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Glycosylation ofα 1 glycoprotein in septic shock: Changes in degree of branching and in expression of sialyl Lewisx groups

  • Glycosylation and Disease Papers
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Abstract

The occurrence of differences in acute-phase response, with respect to concentration and glycosylation of α1-acid glycoprotein (AGP) was studied in the sera of patients surviving or not from septic shock. Crossed affino-immunoelectrophoresis was used with concanavalin A andAleuria aurantia lectin for the detection of the degree of branching and fucosylation, respectively, and the monoclonal CSLEX-1 for the detection of sialyl Lewisx (SLeX) groups on AGP. Septic shock apparently induced an acute-phase response as indicated by the increased serum levels and changed glycosylation of AGP. In the survivor group a transient increase in diantennary glycan content was accompanied by a gradually increasing fucosylation and SLeX expression, comparable to those observed in the early phase of an acute-inflammatory response. Remarkably, in the non-survivor group a modest increase in diantennary glycan content was accompanied by a strong elevation of the fucosylation of AGP and the expression of SLeX groups on AGP, typical for the late phase of an acute-phase response. Our results suggest that these changes in glycosylation of AGP can have a prognostic value for the outcome of septic shock.

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Abbreviations

AAL:

Aleuria aurantia lectin

AGP:

α1-acid glycoprotein

CAIE:

crossed affinoimmunoelectrophoresis

Con A:

Concanavalin A

HSPC:

human serum protein calibrator

IL-1:

interleukin 1

IL-6:

interleukin 6

LIF:

leukaemia inhibitory factor

LPS:

lipopolysaccharide

SLeX:

sialyl Lewisx

TNF:

tumour necrosis factor

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Authors and Affiliations

  1. Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit, van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands

    Els C. M. Brinkman-Van Der Linden, Esther C. R. Van Ommen & Willem Van Dijk

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  1. Els C. M. Brinkman-Van Der Linden
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  2. Esther C. R. Van Ommen
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  3. Willem Van Dijk
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Brinkman-Van Der Linden, E.C.M., Van Ommen, E.C.R. & Van Dijk, W. Glycosylation ofα 1 glycoprotein in septic shock: Changes in degree of branching and in expression of sialyl Lewisx groups. Glycoconjugate J 13, 27–31 (1996). https://doi.org/10.1007/BF01049676

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  • DOI: https://doi.org/10.1007/BF01049676

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