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Increased dopamine D2 receptor-mediated inhibition of [14C]acetylcholine release in the dorsomedial part of the nucleus accumbens

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Abstract

Dopamine (DA) D2 receptor-mediated inhibition of the K+-stimulated release of [14C]acetylcholine (ACh) from prelabeled rat dorsomedial nucleus accumbens slices was found to be 1.7 times greater than that observed in dorsorostral and ventromedial slices. This observation is consistent with the 1.9 fold higher DA D2 receptor density found in the dorsomedial area. In contrast, there were no differences in the DA D2 receptor-mediated effects on [3H]DA release in these areas. In addition, DA D2 receptor-mediated effects on [3H]DA and [14C]ACh release could not be demonstrated in the ventrorostral part of the nucleus accumbens consistent with the fact that DA D2 receptors were barely detectable in this area. The results suggest that cholinergic terminals in the dorsomedial part of the nucleus accumbens are under greater inhibitory DA control than in other areas of the nucleus accumbens.

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References

  1. Willner, P. 1983. Dopamine and depression: A review of recent evidence. II. Theoretical approaches. Brain Res. Revs. 6:225–236.

    Google Scholar 

  2. Fibiger, H. C., and Phillips, A. G. 1981. Increased intracranial self-stimulation in rats after long-term administration of desipramine. Science 214:683–685.

    PubMed  Google Scholar 

  3. Chronister, R. B., Sikes, R. W., Wood, J., and de France, J. F. 1980. The pattern of termination of ventral tegmental afferents into nucleus accumbens: an anterograde HRP analysis. Neurosci. Lett. 17:231–235.

    PubMed  Google Scholar 

  4. Allin, R., Russell, V. A., Lamm, M. C. L., and Taljaard, J. J. F. 1988. Regional distribution of monoamines in the nucleus accumbens of the rat. Neurochem. Res. 13:937–942.

    PubMed  Google Scholar 

  5. Russell, V. A., Lamm, M. C. L., Allin, R., de Villiers, A. S., Searson, A., and Taljaard, J. J. F. 1989. Effect of selective noradrenergic denervation on noradrenaline content and [3H]dopamine release in rat nucleus accumbens slices. Neurochem. Res. 14:169–172.

    PubMed  Google Scholar 

  6. Allin, R., Russell, V. A., Lamm, M. C. L., and Taljaard, J. J. F. 1989. Regional distribution of dopamine D1 and D2 receptors in the nucleus accumbens of the rat. Brain Res. (in press).

  7. Pellegrino, L. J., Pellegrino, A. S., and Cushman, A. J. 1979. A stereotaxic atlas of the rat brain. Pages 10–19, (2nd Ed.) Plenum Press, New York.

    Google Scholar 

  8. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurements with the folin phenol reagent. J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  9. Leysen, J. E., and Gommeren, W. 1981. Optimal conditions for [3H]apomorphine binding and anomalous equilibrium binding of [3H]apomorphine and [3H] spiperone to rat striatal membranes: Involvement of surface phenomena versus multiple binding sites. J. Neurochem. 36:201–219.

    PubMed  Google Scholar 

  10. Munson, P. S., and Rodbard, D. 1980. LIGAND: A versatile computerized approach for characterization of ligand-binding systems. Anal. Biochem. 107:220–239.

    PubMed  Google Scholar 

  11. Hetey, L., and Zimmerman, B. 1986. Characterization of the synaptosomal high-affinity uptake of noradrenaline in the nucleus accumbens of rats. Biomed. Biochim. Acta. 45:1203–1207.

    PubMed  Google Scholar 

  12. Nurse, B., Russell, V. A., and Taljaard, J. J. F. 1985. Effect of chronic desipramine treatment on adrenoceptor modulation of [3H] dopamine release from rat nucleus accumbens slices. Brain Res. 334:235–242.

    PubMed  Google Scholar 

  13. De Langen, C. D. J., Hogenboom, F., and Mulder, A. H. 1979. Presynaptic noradrenergic α-receptors and modulation of 3H-noradrenaline release from rat brain synaptosomes. Eur. J. Pharmacol. 60:79–89.

    PubMed  Google Scholar 

  14. Reyneke, L., Allin, R., Russell, V. A., and Taljaard, J. J. F. 1989. Lack of effect of chronic desipramine treatment on dopaminergic activity in the nucleus accumbens of the rat. Neurochem. Res. 14:661–665.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. MRC Research Unit for the Neurochemistry of Mental Diseases, Department of Chemical Pathology, University of Stellenbosch, Tygerberg Hospital, P.O. Box 113, 7505, Tygerberg, RSA

    V. A. Russell, R. Allin, M. C. L. Lamm & J. J. F. Taljaard

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  1. V. A. Russell
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  2. R. Allin
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  3. M. C. L. Lamm
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  4. J. J. F. Taljaard
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Russell, V.A., Allin, R., Lamm, M.C.L. et al. Increased dopamine D2 receptor-mediated inhibition of [14C]acetylcholine release in the dorsomedial part of the nucleus accumbens. Neurochem Res 14, 877–881 (1989). https://doi.org/10.1007/BF00964818

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  • DOI: https://doi.org/10.1007/BF00964818

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