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Determination of ribonucleosides, deoxyribonucleosides, and purine and pyrimidine bases in adult rabbit cerebrospinal fluid and plasma

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Abstract

Purine and pyrimidine base and nucleoside levels were measured in adult rabbit cisternal CSF and plasma by reversed-phase high-performance liquid chromatography. The concentrations of bases, nucleosides, and nucleoside phosphates were similar in plasma and CSF except for the adenosine phosphates and uracil which were higher in the plasma. In plasma and CSF, adenosine levels were low (0.12 μM) and guanosine, deoxyadenosine, deoxyguanosine, and deoxyinosine were not detectable (<0.1 μM); inosine and xanthine concentrations were 1–2 μM and hypoxanthine concentrations were approximately 5 μM; uridine (∼8 μM), cytidine (2–3 μM), and thymidine, deoxyuridine, and deoxycytidine (0.5–1.4 μM) were easily detectable. In both plasma and CSF, guanine, and thymine were undetectable (<0.1 μM), adenine and cytosine were less than 0.2 μM, but uracil was present (>1 μM). Adenosine, inosine, and guanosine phosphates were also detectable at low concentrations in CSF and plasma. These results are consistent with the hypothesis that purine deoxyribonucleosides are synthesized in situ in the adult rabbit brain. In contrast, pyrimidine deoxyribonucleosides and ribonucleosides, and purine and pyrimidine bases are available in the CSF for use by the brain.

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Abbreviations

HPLC:

high-performance liquid chromatography

AUFS:

absorbance units full scale

CSF:

cerebrospinal fluid

CNS:

central nervous system

ADA:

adenosine deaminase

PNP:

purine nucleoside phosphorylase

XOD:

xanthine oxidase

UC:

uricase

Alk. Phos:

alkaline phosphatase

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Authors and Affiliations

  1. Departments of Medicine and Pharmacology, The University of Iowa College of Medicine, 52242, Iowa City, Iowa

    Janis Thane Eells & Reynold Spector

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  1. Janis Thane Eells
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  2. Reynold Spector
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Eells, J.T., Spector, R. Determination of ribonucleosides, deoxyribonucleosides, and purine and pyrimidine bases in adult rabbit cerebrospinal fluid and plasma. Neurochem Res 8, 1307–1320 (1983). https://doi.org/10.1007/BF00964000

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  • DOI: https://doi.org/10.1007/BF00964000

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