Abstract
The glycosphingolipid compositions of rat mammary tumour cell lines with different metastatic potentials for the lung [a parental tumour cell line (MTC) and its subclones MTLn2 (a non metastatic subclone) and MTLn3 (a subclone with high metastatic potential to the lung)] were studied using a newly developed TLC blotting/secondary ion mass spectrometry system and crude glycosphingolipids obtained from 0.5−1×107 cells of each cell line. GM3 and GM2 were the major components of the MTC cell line, but they were very minor components in the MTLn2 and MTLn3 cell lines, GDla being the major ganglioside. HexNAc-fucosyl-GMla was found in the MTLn2 cells by the TLC blotting/SIMS method, and the terminal sugar linkage was shown to be a blood group A-type structure by immunostaining. These findings suggest that the ganglioside is a novel type of blood group A-active ganglioside, GalNAcα1-3(Fucα1-2)GMla. No blood group A-active lipid was present in MTLn3 cells, whereas Hex-GMla and neutral glycosphingolipids with more than 5 sugar residues were.
Similar content being viewed by others
References
Svennerholm L (1963)J Neurochem 10: 613–23.
Kojima N, Hakomori S (1989)J Biol Chem 264: 20159–62.
Zheng M, Tsuruoka T, Tsuji T, Hakomori S (1992)Biochem Biophys Res Commun 186: 1397–1402.
Phillips ML, Nudelman E, Gaeta FC, Perez M, Singhal AK, Hakomori S, Paulson JC (1990)Science 250: 1130–32.
Hakomori S (1981)Annu Rev Biochem 50: 733–64.
Hakomori S (1989)Cancer Res 52: 257–331.
Taki T, Takamatsu M, Myoga A, Tanaka K, Ando S, Matsumoto M (1988)J Biochem 103: 998–1003.
Taki T, Yamamoto K, Takamatsu M, Ishii K, Myoga A, Sekiguchi K, Ikeda I, Kurata K, Nakajima J, Handa S, Matsumoto M (1990)Cancer Res 50: 1284–90.
Hakomori S, Kannagi R (1983)J Natl Cancer Inst 71: 231–51.
Taki T, Kawamoto M, Seto H, Noro N, Masuda T, Kannagi R, Matsumoto M (1983)J Biochem 94: 633–44.
Neri A, Welch DR, Kawaguchi T, Nicorson G (1983)J Natl Cancer Inst 68: 507–17.
Taki T, Ishikawa D, Handa S, Kasama T (1995)Anal Biochem 225: 24–27.
Saito T, Hakomori S (1971)J Lipid Res 12: 257–59.
Taki T, Handa S, Ishikawa D (1994)Anal Biochem 221: 312–16.
Taki T, Kasama T, Handa S, Ishikawa D (1994)Anal Biochem 223: 232–38.
Mansson JE, Egge MME, Svennerholm L (1986)FEBS Lett 196: 259–62.
Taki T, Rokukawa C, Kasama T, Kon K, Ando S, Abe T, Handa S (1992)J Biol Chem 267: 11811–17.
Taki T, Rokukawa C, Kasama T, Handa S (1992)Cancer Res 52: 4805–11.
Author information
Authors and Affiliations
Additional information
Abbreviations: TLC, thin-layer chromatography; HPTLC plate, high performance thin-layer chromatography-plate; PVDF, polyvinylidene difluoride; SIMS, secondary ion mass spectrometry; GC-MS, gas chromatography-mass spectrometry; C16:0, hexadecanoic acid; C18:0, octadecanoic acid; C22:0, docosanoic acid; C24:0, tetracosanoic acid; d18:1, 2-amino-4-octadecene-1,3-diol; Hex, hexose; HexNAc,N-acetylhexosamine; Gal, galactose; Glc, glucose; GalNAc,N-acetylgalactosamine; Lac, lactose; NeuAc,N-acetylneuraminic acid; Cer, ceramide; Glob, globoside; iGlob, isogloboside; GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb3Cer, Galα1-4Galβ1-4Glcβ1-1Cer; Gb4Cer (Glob), GalNAcβ1-3Galα1-4Glcβ1-1Cer; iGb3Cer, Galα1-3Galβ1-4Glcβ1-1Cer; iGb4Cer (iBlob), GalNAcβ1-3Galβ1-4Glcβ1-1Cer; Ganglio-series gangliosides are named according to Svennerholm [1].
Rights and permissions
About this article
Cite this article
Kasama, T., Hisano, Y., Nakajima, M. et al. Microscale analysis of glycosphingolipids by TLC blotting/secondary ion mass spectrometry: a novel blood group A-active glycosphingolipid and changes in glycosphingolipid expression in rat mammary tumour cells with different metastatic potentials. Glycoconjugate J 13, 461–469 (1996). https://doi.org/10.1007/BF00731479
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00731479