Skip to main content
Log in

Microscale analysis of glycosphingolipids by TLC blotting/secondary ion mass spectrometry: a novel blood group A-active glycosphingolipid and changes in glycosphingolipid expression in rat mammary tumour cells with different metastatic potentials

  • Papers Dedicated To Dr Sen-Itiroh Hakomori
  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

The glycosphingolipid compositions of rat mammary tumour cell lines with different metastatic potentials for the lung [a parental tumour cell line (MTC) and its subclones MTLn2 (a non metastatic subclone) and MTLn3 (a subclone with high metastatic potential to the lung)] were studied using a newly developed TLC blotting/secondary ion mass spectrometry system and crude glycosphingolipids obtained from 0.5−1×107 cells of each cell line. GM3 and GM2 were the major components of the MTC cell line, but they were very minor components in the MTLn2 and MTLn3 cell lines, GDla being the major ganglioside. HexNAc-fucosyl-GMla was found in the MTLn2 cells by the TLC blotting/SIMS method, and the terminal sugar linkage was shown to be a blood group A-type structure by immunostaining. These findings suggest that the ganglioside is a novel type of blood group A-active ganglioside, GalNAcα1-3(Fucα1-2)GMla. No blood group A-active lipid was present in MTLn3 cells, whereas Hex-GMla and neutral glycosphingolipids with more than 5 sugar residues were.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Svennerholm L (1963)J Neurochem 10: 613–23.

    Google Scholar 

  2. Kojima N, Hakomori S (1989)J Biol Chem 264: 20159–62.

    Google Scholar 

  3. Zheng M, Tsuruoka T, Tsuji T, Hakomori S (1992)Biochem Biophys Res Commun 186: 1397–1402.

    Google Scholar 

  4. Phillips ML, Nudelman E, Gaeta FC, Perez M, Singhal AK, Hakomori S, Paulson JC (1990)Science 250: 1130–32.

    Google Scholar 

  5. Hakomori S (1981)Annu Rev Biochem 50: 733–64.

    Google Scholar 

  6. Hakomori S (1989)Cancer Res 52: 257–331.

    Google Scholar 

  7. Taki T, Takamatsu M, Myoga A, Tanaka K, Ando S, Matsumoto M (1988)J Biochem 103: 998–1003.

    Google Scholar 

  8. Taki T, Yamamoto K, Takamatsu M, Ishii K, Myoga A, Sekiguchi K, Ikeda I, Kurata K, Nakajima J, Handa S, Matsumoto M (1990)Cancer Res 50: 1284–90.

    Google Scholar 

  9. Hakomori S, Kannagi R (1983)J Natl Cancer Inst 71: 231–51.

    Google Scholar 

  10. Taki T, Kawamoto M, Seto H, Noro N, Masuda T, Kannagi R, Matsumoto M (1983)J Biochem 94: 633–44.

    Google Scholar 

  11. Neri A, Welch DR, Kawaguchi T, Nicorson G (1983)J Natl Cancer Inst 68: 507–17.

    Google Scholar 

  12. Taki T, Ishikawa D, Handa S, Kasama T (1995)Anal Biochem 225: 24–27.

    Google Scholar 

  13. Saito T, Hakomori S (1971)J Lipid Res 12: 257–59.

    Google Scholar 

  14. Taki T, Handa S, Ishikawa D (1994)Anal Biochem 221: 312–16.

    Google Scholar 

  15. Taki T, Kasama T, Handa S, Ishikawa D (1994)Anal Biochem 223: 232–38.

    Google Scholar 

  16. Mansson JE, Egge MME, Svennerholm L (1986)FEBS Lett 196: 259–62.

    Google Scholar 

  17. Taki T, Rokukawa C, Kasama T, Kon K, Ando S, Abe T, Handa S (1992)J Biol Chem 267: 11811–17.

    Google Scholar 

  18. Taki T, Rokukawa C, Kasama T, Handa S (1992)Cancer Res 52: 4805–11.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Abbreviations: TLC, thin-layer chromatography; HPTLC plate, high performance thin-layer chromatography-plate; PVDF, polyvinylidene difluoride; SIMS, secondary ion mass spectrometry; GC-MS, gas chromatography-mass spectrometry; C16:0, hexadecanoic acid; C18:0, octadecanoic acid; C22:0, docosanoic acid; C24:0, tetracosanoic acid; d18:1, 2-amino-4-octadecene-1,3-diol; Hex, hexose; HexNAc,N-acetylhexosamine; Gal, galactose; Glc, glucose; GalNAc,N-acetylgalactosamine; Lac, lactose; NeuAc,N-acetylneuraminic acid; Cer, ceramide; Glob, globoside; iGlob, isogloboside; GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb3Cer, Galα1-4Galβ1-4Glcβ1-1Cer; Gb4Cer (Glob), GalNAcβ1-3Galα1-4Glcβ1-1Cer; iGb3Cer, Galα1-3Galβ1-4Glcβ1-1Cer; iGb4Cer (iBlob), GalNAcβ1-3Galβ1-4Glcβ1-1Cer; Ganglio-series gangliosides are named according to Svennerholm [1].

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasama, T., Hisano, Y., Nakajima, M. et al. Microscale analysis of glycosphingolipids by TLC blotting/secondary ion mass spectrometry: a novel blood group A-active glycosphingolipid and changes in glycosphingolipid expression in rat mammary tumour cells with different metastatic potentials. Glycoconjugate J 13, 461–469 (1996). https://doi.org/10.1007/BF00731479

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00731479

Keywords

Navigation