Skip to main content
SpringerLink
Log in

Electrogenic Na pump evidenced by injecting various Na salts into the isolated A-V node cells of rabbit heart

  • Excitable Tissues and Central Nervous Physiology
  • Letters and Notes
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

Electrogenicity of the Na pump was confirmed by injecting Na salts into a small cluster of A-V node cells. Injection of Na glutamate or Na acetate induced marked hyperpolarization, accompanying with cessation of spontaneous activity. The hyperpolarization exceeded EK in 27 mM K Tyrode solution and was inhibited by 10−5 M strophanthidin. Injection of NaCl or NaI depolarized the membrane. These data showed that inward-going current carried by the injected anion antagonized the outward-going pump current and thus determined the net effect of the injection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Christoffersen GRJ (1972) Steady state contribution of the Na,K-pump to the membrane potential in identified neurons of a terrestrial snail, Helix pomatia. Acta physiol scand 86: 498–514

    Google Scholar 

  • Eccles SJ, Eccles RM, Ito M (1964) Effects produced on inhibitory postsynsptic potentials by the coupled injections of cations and anions into motoneurons. Proc Roy Soc B 160: 197–210

    Google Scholar 

  • Glitsch HG (1979) Characteristics of active Na transport in intact cardiac cells. Am J Physiol 236 (2): H189-H199

    Google Scholar 

  • Kerkut GA, Thomas RC (1965) An electrogenic sodium pump in snail neurons. Comp Biochem Physiol 14: 167–183

    Google Scholar 

  • Kurachi Y, Noma A, Irisawa H (1981) Electrogenic sodium pump in rabbit A-V node cell. accepted for publication. Pflűgers Arch.

  • Lee CO, Fozzard HA (1979) Membrane permeability during low potassium depolarization in sheep cardiac Purkinje fibers. Am J Pysiol 237 (3): C156-C165

    Google Scholar 

  • Rang HP, Ritchie JM (1968) On the electrogenic sodium pump in mammalian non-myelinated nerve fibres and its activation by various external cations. J Physiol (Lond) 196: 183–221

    Google Scholar 

  • Taniguchi J, Kokubun S, Noma A, Irisawa H (1981) Spontaneously active cells isolated from the sino-atrial and atrio-ventricular nodes of the rabbit heart. Jpn J Physiol 31: 547–558

    Google Scholar 

  • Thomas RC (1972) Electrogenic sodium pump in nerve and muscle cells. Phsiol Rev 52: 563–594

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Physiological Sciences, Myodaiji, 444, Okazaki, Japan

    Y. Kurachi, A. Noma & H. Irisawa

Authors
  1. Y. Kurachi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Noma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Irisawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kurachi, Y., Noma, A. & Irisawa, H. Electrogenic Na pump evidenced by injecting various Na salts into the isolated A-V node cells of rabbit heart. Pflugers Arch. 392, 89–91 (1981). https://doi.org/10.1007/BF00584587

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00584587

Key words

Search

Navigation