Summary
Electrical stimulation of the preganglionic sympathetic neurons rapidly and markedly elevated the contents of the primary dopamine (DA) and noradrenaline (NA) metabolites, i.e., 3,4-dihydroxyphenylacetic acid (DOPAC) and 3,4-dihydroxyphenylethylene glycol (DOPEG) in the superior cervical ganglion and it enhanced the accumulation of DOPAC and DA following inhibition of the DA-β-hydroxylase by FLA-63. The stimulation also increased the concentration of DA and decreased the concentration of NA in the salivary gland both without and with DA-β-hydroxylase inhibition. Chlorisondamine inhibited the increase in ganglionic DOPAC following preganglionic stimulation (5 Hz, 30 min) by 30–50% and it even better reduced the biochemical changes in the salivary gland. Atropine did not produce any clearcut inhibition of the stimulation-induced effects on the superior cervical ganglion or the salivary gland, nor did it enhance the effect of chlorisondamine. The results suggest that nicotine, but not muscarine receptors in the cell body region of the postganglionic NA neurons partially mediate the effects of preganglionic stimulation. The effects remaining after blockade of the nicotine and muscarine receptors might be due to release of a neuropeptide acting on a special receptor. The stimulation-induced increase in the concentration of DOPAC in the superior cervical ganglion might, at least partly, be the result of a depolarization of the NA nerve cell body regions since similar changes were produced by electrical stimulation of the chronically decentralized ganglion.
Similar content being viewed by others
References
Andén N-E, Golembiowska-Nikitin K, Thornström U (1982) Selective stimulation of dopamine and noradrenaline autoreceptors by B-HT 920 and B-HT 933, respectively. Naunyn-Schmiedeberg's Arch Pharmacol 321:100–104
Andén N-E, Grabowska-Andén M (1985) Synthesis and utilization of catecholamines in the rat superior cervical ganglion following changes in the nerve impulse flow. J Neural Transm 64:81–92
Andén N-E, Grabowska-Andén M, Lindgren S, Oweling M (1985) Very rapid turnover of dopamine in noradrenaline cell body regions. Naunyn-Schmiedeberg's Arch Pharmacol 329:258–263.
Bhatnagar RK, Moore KE (1971) Effects of electrical stimulation, α-methyltyrosine and desmethylimipramine on the norepinephrine contents of neuronal cell bodies and terminals. J Pharmacol Exp Ther 178:450–463
Carlsson A, Lindqvist M (1974) Studies on the neurogenic short-term control of adrenomedullary hormone synthesis. J Neural Transm 35:181–196
Chalazonitis A, Rice PJ, Zigmond RE (1980) Increased ganglionic tyrosine hydroxylase and dopamine-β-hydroxylase activities following preganglionic nerve stimulation: role of nicotinic receptors. J Pharmacol Exp Ther 213:139–143
Felice LJ, Felice JD, Kissinger PT (1978) Determination of catecholamines in rat brain parts by reverse-phase ion-pair liquid chromatography. J Neurochem 31:1461–1465
Fredholm B, Sedvall G (1966) Influence of sympathetic nerve stimulation of noradrenaline stores in the rat salivary gland. Life Sci 5:2023–2032
Ip NY, Perlman RL, Zigmond RE (1983) Acute transsynaptic regulation of tyrosine 3-monooxygenase activity in the rat supcrior cervical ganglion: evidence for both cholinergic and noncholinergic mechanisms. Proc Nat Acad Sci USA 80:2081–2085
Jan YN, Jan LY, Kuffler SW (1979) A peptide as a possible transmitter in sympathetic ganglia of the frog. Proc Nat Acad Sci USA 76:1501–1505
Konishi S, Otsuka M, Folkers K, Rosell S (1983) A substance P antagonist blocks non-cholinergic slow excitatory postsynaptic potential in guinea-pig sympathetic ganglia. Acta Physiol Scand 117:157–160
Mueller RA, Thoenen H, Axelrod J (1970) Inhibition of neuronally induced tyrosine hydroxylase by nicotinic receptor blockade. Eur J Pharmacol 10:51–56
Mytilineou C, Black IB (1976) Regeneration of sympathetic neurons: effect of decentralization. Brain Res 109:382–386
Otten U, Thoenen H (1976) Mechanisms of tyrosine hydroxylase and dopamineβ-hydroxylase induction in organ cultures of rat sympathetic ganglia by potassium depolarization and cholinomimetics. Naunyn-Schmiedeberg's Arch Pharmacol 292:153–159
Snider SR, Almgren O, Carlsson A (1973) The occurrence and functional significance of dopamine in some peripheral adrenergic nerves of the rat. Naunyn-Schmiedeberg's Arch Pharmacol 278:1–12
Snider SR, Carlsson A (1972) The adrenal dopamine as an indicator of adrenomedullary hormone biosynthesis. Naunyn-Schmiedeberg's Arch Pharmacol 275:347–357
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Andén, NE., Grabowska-Andén, M. & Klaesson, L. Stimulation of the synthesis of catecholamines in a sympathetic ganglion via cholinergic and non-cholinergic mechanisms. Naunyn-Schmiedeberg's Arch. Pharmacol. 333, 17–22 (1986). https://doi.org/10.1007/BF00569654
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00569654