Abstract
Two-dimensional gel electrophoresis of hepatic microsomes from phenobarbital-treated animals was used to analyze electrophoretic/regulatory polymorphisms for cytochromes P-450b, P-450e, P-450g, and P-450h in 28 inbred strains of rat. Previous studies with outbred rats revealed the existence of four electrophoretic variants for P-450b, two for P-450e, and three for P-450h as well as two regulatory alleles for P-450g. With the exception of one allozymic form of P-450h, all of these alleles as well as a novel (null) allele for P-450e were found to be homozygous in at least two of the inbred strains tested. Eight phenotypes for combinations of these four cytochromes P-450 were observed. Inbred strains were identified that can be used in studies on the structure/function of unique cytochrome P-450-allozymes and in genetic crosses to map the four distinct cytochrome P-450 genes.
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This work was supported by National Institutes of Health Biomedical Research Support Grant SO7RR07208.
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Rampersaud, A., Walz, F.G. Polymorphisms of four hepatic cytochromes P-450 in twenty-eight inbred strains of rat. Biochem Genet 25, 527–534 (1987). https://doi.org/10.1007/BF00554354
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DOI: https://doi.org/10.1007/BF00554354