Summary
Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei. In this staining procedure, the first antigen was localized using 3,3′-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphtol basic dye (2-NBD) method. The two antigens were localized in different cells or structures. No overlap of the staining was observed, thus indicating that dopamine is not localized with serotonin, 5-methoxytryptamine or tryptamine.
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Geffard, M., Tuffet, S., Mons, N. et al. Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies. Histochemistry 88, 61–64 (1987). https://doi.org/10.1007/BF00490169
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DOI: https://doi.org/10.1007/BF00490169