Summary
Prostaglandins are considered to play important roles in gastric mucosal protection. The rate-limiting enzyme involved in the biosynthesis of prostaglandins is cyclooxygenase (COX), also known as prostaglandin H synthase. Two forms of COX are known: a constitutively expressed form (COX-1) and a newly-characterized, inducible form (COX-2). In the present study, the immunocytochemical localization of COX-1 and COX-2 was examined in the rat gastrointestinal tract. A strong immunoreactivity for COX-1 was localized in the mucous neck cells of gastric gland. A weak reactivity for COX-1 was also found in the mucous cell types in the cardiac gland and pyloric gland of the stomach as well as in the Brunner's gland of duodenum. Ultrastructurally, the immunoreactivity was localized to the apical cytoplasm of these cells. On the other hand, immunoreactivity for COX-2 was distributed in the surface mucous cells in both the fundic and pyloric regions of stomach. These results suggest that a subset of mucous cells is the primary site for production of prostaglandins in the rat gastrointestinal tract, and that two forms of COX are expressed in distinct types of mucous cell.
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References
Bennett, A., Stamford, I. F. & Unger, W. G. (1973) Prostaglandin E2 and gastric acid secretion in man. J. PhysioL. 229, 349–60.
Dewitt, D. & Smith, W. (1989) Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence. Proc. Natl Acad. Sci. USA 85, 1412–16.
Fujimoto, Y., Nakatani, E., Minamino, I., Takahashi, M., Sakuma, S. & Fujita, T. (1990) Effective use of rabbit gastric antral mucosal slices in prostaglandin synthesis and metabolism studies. Biochim. Biophys. Acta 1044, 65–9.
Johansson, C. & Bergstrom, S. (1982) Prostaglandins and protection of the gastroduodenal mucosa. Scand. J. GastroenteroL. 77 (suppl.), 21–46.
Katsuyama, T. & Spicer, S. S. (1978) Histochemical differentiation of complex carbohydrates with variants of the concanavalin A-horseradish peroxidase method. J. Histochem. CytocheM. 26, 233–50.
Kobayashi, K., Higuchi, K., Arakawa, T., Matsumoto, T. & Nagura, I. (1992) Effect of sofalcone on localization of 15-hydroxyprostaglandin dehydrogenase, an enzyme that metabolizes prostaglandin E2 in rat gastric mucosa: an immunohistochemical study. J. Clin. GastroenteroL. 14 (suppl. 1), 39–42.
Konturek, S. J., Radecki, T., Brzozowski, T., Piastucki, I., Dembinska-Kiec, A. & Zmuda, A. (1967) Gastric cytoprotection by prostaglandins, ranitidine, and probanthine in rats. Role of endogenous prostaglandins. Scand. J. GastroenteroL. 16, 7–12.
Kujubu, D. A., Fletcher, B. S., Varnum, B. C., Lim, R. W. & Herschman, H. R. (1991) TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J. Biol. CheM. 266, 12 866–72.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–5.
O'Neill, G. P. & Ford-Huchinson, A. W. (1993) Expression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues. FEBS LetT. 330, 156–60.
Robert, A., Nezamis, J. E. & Philips, J. P. (1967) Inhibition of gastric secretion by prostaglandins. Am. J. Dig. DiS. 12, 1073–6.
Robert, A., Nezamis, J. E., Lancaster, C. & Hanchar, A. J. (1979) Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 77, 433–43.
Rollins, T. E. & Smith, W. L. (1980) Subcellular localization of prostaglandin-forming cyclooxygenase in Swiss mouse 3T3 fibroblasts by electron microscopic immunocytochemistry. J. Biol. CheM. 255, 4872–5.
Roth, G. J., Siok, C. J. & Ozols, J. (1980) Structural characteristics of prostaglandin synthase from sheep vesicular gland. J. Biol. CheM. 255, 1301–4.
Seidler, U., Beinborn, M. & Sewing, K-F. (1989) Inhibition of acid formation in rabbit parietal cells by prostaglandins is mediated by the prostaglandin E2 receptor. Gastroenterology 96, 314–20.
Sirois, J. & Richards, J. S. (1992) Purification and characterization of a novel, distinct isoform of prostaglandin endoperoxide synthase induced by human chorionic gonadotropin in granulosa cells of rat preovulatory follicles. J. Biol. CheM. 267, 6382–8.
Smith, W. L. & Marnett, L. J. (1991) Prostaglandin endoperoxide synthase: structure and catalysis. Biochim. Biophys. Acta 1083, 1–17.
Suganuma, T., Katsuyama, T., Tsukahara, M., Tatematsu, M., Sakakura, Y. & Murata, F. (1981) Comparative histochemical study of alimentary tracts with special reference to the mucous neck cells of the stomach. Am. J. AnaT. 161, 219–38.
Towbin, I., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci. USA 76, 4350–4.
Xie, W., Chipman, J. G., Robertson, D. L., Erikson, R. L. & Simmons, D. L. (1991) Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc. Natl Acad. Sci. USA 88, 2692–6.
Yokoyama, C. & Tanabe, T. (1989) Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem. Biophys. Res. CommuN. 165, 888–94.
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Iseki, S. Immunocytochemical localization of cyclooxygenase-1 and cyclooxygenase-2 in the rat stomach. Histochem J 27, 323–328 (1995). https://doi.org/10.1007/BF00398975
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DOI: https://doi.org/10.1007/BF00398975