Abstract
The beta adrenergic (βAR) and muscarinic (MR) receptors have been quantitated in parallel, using 125I-pindolol and 3H-quinuclidinylbenzilate, in a model of compensatory left ventricular (LV) hypertrophy (LVH), which developed in rats 4–6 weeks after an abdominal aortic stenosis. Since aortic banding resulted in a pronounced LVH of 62%, the results were expressed both in terms of density (fmol/mg protein) and quantity (fmol per LV). In addition, competition curves using either a specific β1-antagonist or isoproterenol or carbachol allowed the determination of the two βAR subtypes and of the low and high affinity sites (defined by the inhibitory constant K i) for both β1AR and MR. In LVH, receptor density decreased for each of total βAR, β1AR subtype, high affinity (K i 6–8nM) β1AR sites (from 26±2 to 19±3 fmol/mg protein, P<0.05), total MR and high affinity (K i 12 nM) MR sites (from 63±6 to 40±4 fmol/mg protein, P<0.001). The βAR and MR densities dropped in parallel so that the MR/βAR ratio remained unchanged. In sharp contrast (because the LVs were bigger) the quantities of total βAR, β1 AR subtype, β1AR high affinity sites, total MR and MR high affinity sites per LV were unmodified. Thus, in compensatory LVH the total quantity of receptors per LV was unchanged, suggesting that either both synthesis and degradation were augmented in parallel or that during the process of cardiac hypertrophy they are not regulated, i. e., that the genes encoding for the two receptors were not activated, as it has been suggested for other membrane proteins. Assuming that the high affinity sites represent the receptors coupled to the adenylyl cyclase, we also propose that the overall regulatory systems were modified beyond the level of the receptors.
Similar content being viewed by others
References
Anversa P, Loud AV, Giacomelli F, Weiner J (1978) Absolute morphometric study of myocardial hypertrophy in experimental hypertension. II. Ultrastructure of myocytes and interstitium. Lab Invest 38:597–600
Baker KM, Chemin MI, Wixson SK, Aceto JF (1990) Reninangiotensin system involvement in pressure-overload cardiac hypertrophy in rats. Am J Physiol 259:H324-H332
Bastie D de la, Levitsky D, Rappaport L, Mercadier JJ, Marotte F, Winewsky C, Brovkovich V, Schwartz K, Lompré AM (1990) Function of the sarcoplasmic reticulum and expression of its Ca2+-ATPase gene in pressure overload-induced cardiac hypertrophy in the rat. Circ Res 66:554–564
Bilski AJ, Halliday SE, Fitzgerald JD, Wale JL (1983) The pharmacology of a β2-selective adrenoceptor antagonist (ICI 118,551). J Cardiovasc Pharmacol 5:430–437
Bristow MR, Ginsburg R, Umans V, Fowler M, Minobe W, Rasmussen R, Zera P, Billingham ME, Harrison DC, Stinson EB (1986) β1 and β2 adrenergic receptor subpopulation in non failing and failing human left ventricular myocardium: coupling of both receptor subtypes to muscle contraction and selective β1 receptor down-regulation in heart failure. Circ Res 59:297–309
Brodde OE (1991) β1-and β2- adrenoceptors in the human heart: properties, function, and alterations in chronic heart failure. Pharmacol Rev 43:203–243
Callens-El Amrani E, Mayoux E, Mouas C, Ventura-Clapier R, Henzel D, Charlemagne D, Swynghedauw B (1990) Normal responsiveness to external calcium and to calcium channel modifying agents in hypertrophied rat heart. Am J Physiol 258:H1727-H1734
Casey PJ, Gilman AG (1988) G protein involvement in receptor-effector coupling. J Biol Chem 263:2577–2580
Chen L, Vatner DE, Vatner SF, Hittinger L, Homey CJ (1991) Decreased Gsα mRNA levels accompany the fall in Gs and adenylyl cyclase activities in compensated left ventricular hypertrophy. J Clin Invest 87:293–298
Chevalier B, Swynghedauw B (1990) The β-adrenergic receptors in hypertrophy and heart failure. In: Swynghedauw B (ed) Research in cardiac hypertrophy and failure. INSERM/Libbey, London, pp 201–216
Chevalier B, Mansier P, Callens F, Swynghedauw B (1989) β-Adrenergic system is modified in compensatory pressure cardiac overload in rats: physiological and biochemical evidence. J Cardiovasc Pharmacol 13:412–420
Crozatier B, Bo Su J, Corsin A, Bouanani NH (1991) Species differences in myocardial β-adrenergic receptor regulation in response to hyperthyroidism. Circ Res 69:1234–1243
Feldman AM, Cates AE, Bristow MR, Dop C van (1989) Altered expression of α-subunits of G proteins in failing human heart. J Mol Cell Cardiol 21:359–365
Fields JZ, Roeske WR, Morkin E, Yamamura HI (1978) Cardiac muscarinic cholinergic receptors. J Biol Chem 253: 3251–3258
Foster KA, Hock CE, Reibel DK (1991) Altered responsiveness of hypertrophied rat hearts to alpha-and beta-adrenergic stimulation. J Mol Cell Cardiol 23:91–101
Ganguly PK, Sherwood GR (1991) Noradrenaline turn-over and metabolism in myocardium following aortic constriction in rats. Cardiovasc Res 25:579–585
Gende OA, Mattiazzi A, Camillon MC, Pedroni P, Taquini C, Lambi HG, Cincolani HE (1985) Renal hypertension impairs inotropic isoproterenol effect without β-receptor changes. Am J Physiol 249:H814-H819
Goyal RK (1989) Muscarinic receptors subtypes: Physiology and clinical implications. N Engl J Med 321:1022–1029
Kumano K, Upsher ME, Khairallah PA (1983) Beta adrenergic receptor response coupling in hypertrophied hearts. Hypertension 5:I–175-I–183
Laurent GJ, Millward DJ (1980) Protein turn-over during skeletal muscle hypertrophy. Fed Proc 39:42–47
Limas CJ (1979) Increased number of β-adrenergic receptors in the hypertrophied myocardium. Biochim Biophys Acta 588:174–178
Mazancourt P de, Lacasa D, Giot J, Giudicelli Y (1989) Mechanism of the increased adenosine antilipolytic effect in rat fat cells after adrenalectomy: role of intracellular cyclic AMP and the Ri-receptor-Gi coupled adenylate cyclase inhibitory pathway. Endocrinology 124:1131–1139
Morkin E, Kimata S, Skillman JJ (1972) Myosin synthesis and degradation during development of cardiac hypertrophy in the rabbit. Circ Res 30:690–702
Munson PJ, Rodbard D (1980) Ligand, a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem 107:220–239
Musgrave IF, Foucart S, Majewski H (1991) Evidence that angiotensin II enhances noradrenaline release from sympathetic nerves in mouse atria by activating protein kinase C. J Auton Pharmacol 11:211–220
Scamps F, Mayoux E, Charlemagne D, Vassort G (1990) Calcium current in single cells isolated from normal and hypertrophied rat heart. Circ Res 67:199–208
Schnabel P, Böhm M, Gierschik P, Jakobs KH, Erdmann E (1990) Improvement of cholera toxin-catalyzed ADP-ribosylation by endogenous ADP-ribosylation factor from bovine brain provides evidence for an unchanged amount of Gsα in the failing human myocardium. J Mol Cell Cardiol 22:73–82
Stiles GL, Caron MG, Lefkowitz RJ (1984) β-adrenergic receptors: biochemical mechanisms of physiological regulation. Physiol Rev 64:661–743
Swynghedauw B (1989) Remodelling of the heart in response to chronic mechanical overload. Eur Heart J 10:935–939
Upsher ME, Khairallah PA (1985) Beta-adrenergic receptors in rat myocardium during the development and reversal of hypertrophy and following chronic infusions of angiotensin II and epinephrine. Arch Int Pharmacodyn Ther 274:65–79
Vatner DE, Homcy CJ, Sit SP, Manders WT, Vatner SF (1984) Effects of pressure overload left ventricular hypertrophy on beta-adrenergic receptors and responsiveness to catecholamines. J Clin Invest 73:1473–1482
Vatner DE, Lee DL, Schwartz KR, Longabaugh JP, Fuji AM, Stephen FV, Vatner CJ (1988) Impaired cardiac muscarinic receptor function in dog with heart failure. J Clin Invest 81:1836–1842
Vatner DE, Vatner SF, Homcy CJ (1985) Loss of high affinity cardiac beta adrenergic receptors in dogs with heart failure. J Clin Invest 76:2259–2264
Will-Shahab L, Kuttner I, Warbanow W (1986) Signal transfer in cardiac muscle: Alteration of the β-adrenoceptor adenylate cyclase system in the hypertrophied myocardium. Biomed Biochim Acta 45: S199-S204
Woodcock EA, Funder JW, Johnson CI (1985) Decreased cardiac β-adrenergic receptors in deoxycortisone-salt and renal hypertensive rats. Circ Res 45:560–565
Zar JH (1984) Multisample hypotheses: analysis of variance. In: Zar JH (ed) Biostatistical analysis. Prentice Hall, Englewood Cliffs, N. J., pp 162–184
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mansier, P., Chevalier, B., Barnett, D.B. et al. Beta adrenergic and muscarinic receptors in compensatory cardiac hypertrophy of the adult rat. Pflügers Arch. 424, 354–360 (1993). https://doi.org/10.1007/BF00384363
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00384363