Summary
To clarify whether activity of the sympathetic nervous system is decreased in streptozotocin-induced diabetic rats, noradrenaline turnover, which is a reliable indicator of sympathetic nervous system activity, was measured in the interscapular brown adipose tissue, heart and pancreas of streptozotocin diabetic rats. Results from studies using inhibition of noradrenaline biosynthesis with α-methyl-p-tyrosine demonstrated significant reductions (p<0.05-0.001) in sympathetic nervous system activity in the interscapular brown adipose tissue, heart and pancreas of streptozotocin (65 mg/kg) diabetic rats, compared with measurements in streptozotocin (35 mg/kg) diabetic and saline-control rats. The daily injections of neutral protamine Hagedorn insulin to streptoz/otocin (65 mg/kg) diabetic rats prevented the decrease of noradrenaline turnover in the interscapular brown adipose tissue and heart significantly (p<0.02), but this was less marked in pancreas, compared with non-treated streptozotocin (65 mg/kg) diabetic rats. Furthermore reduced noradrenaline turnover was also observed in the control rats which showed comparable changes in body weight to the rats injected with streptozotocin (65 mg/kg). These results suggest that poorly controlled streptozotocin diabetic rats may have reduced sympathetic nervous function, and that insulin therapy might prevent this.
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References
Christensen NJ (1979) Catecholamine and diabetes mellitus. Diabetologia 16: 211–224
Christensen NJ (1972) Plasma catecholamines in long-term diabetics with and without neuropathy and in hypophysectomized subjects. J Clin Invest 51: 779–787
Christensen NJ (1983) Acute effects of insulin on cardiovascular function and noradrenaline uptake and release. Diabetologia 25: 377–381
Neubauer B, Christensen NJ (1976) The noradrenaline, adrenaline and dopamine content in the cardiovascular system in longterm diabetics. Diabetes 25: 6–10
Gundersen HJG, Neubauer B (1977) A long-term diabetic autonomie nervous abnormality. Reduced variations in resting heart rate measured by a simple and sensitive method. Diabetologia 13: 137–140
Wheele T, Watkins PJ (1973) Cardiac denervation in diabetes. Br Med J 4: 584–586
Gundersen HJG (1974) An abnormality of the central autonomic nervous system in long-term diabetes: absence of hippus. Diabetologia 10: 366 (Abstract)
Young JB, Landsberg L (1979) Sympathoadrenal activity in fasting pregnant rats: Dissociation of adrenal medullary and sympathetic nervous system responses. J Clin Invest 64: 109–116
Rappaport EB, Young JB, Landsberg L (1982) Effect of 2-deoxy-D-glucose on the cardiac sympathetic nerves and the adrenal medulla in the rat: further evidence for a dissociation of sympathetic nervous system and adrenal medullary responses. Endocrinology 110: 650–656
Young JB, Fish S, Landsberg L (1983) Sympathetic nervous system and adrenal medullary responses to ischemic injury in mice. Am J Physiol 245: E67-E73
Young JB, Rosa RM, Landsberg L (1984) Dissociation of sympathetic nervous system and adrenal medullary responses. Am J Physiol 247: E35-E40
Spector S, Sjoerdsma A, Udenfriend S (1965) Blockade of endogenous norepinephrine synthesis by alpha-methyl-tyrosine, an inhibition of tyrosine hydroxylase. J Pharmacol Exp Ther 147: 86–95
Young JB, Landsberg L (1977) Stimulation of sympathetic nervous system during sucrose feeding. Nature (London) 269: 615–617
Young JB, Landsberg L (1977) Suppression of sympathetic nervous system during fasting. Science 196: 1473–1475
Avakian EV, Horvath SM (1981) Starvation suppresses sympathoadrenal medullary response to cold exposure in rats. Am J Physiol 241: E316-E320
Yoshida T, Kemnitz JW, Bray GA (1983) Lateral hypothalamic lesions and norepinephrine turnover in rats. J Clin Invest 72: 919–927
Mirsky IA (1973) Insulin: purification and biochemical characterization. In: Berson SA (ed) Methods in investigative and diagnostic endocrinology, Vol2B. American Elsevier, New York, pp 823–883
Peuler JD, Johnson GA (1977) Simultaneous single isotope radioenzymatic assay of plasma norepinephrine, epinephrine and dopamine. Life Sci 21: 625–636
Hales CN, Randle PJ (1963) Immunoassay of insulin with insulin antibody precipitate. Biochem J 88: 137–146
Zar JH (1974) Biostatistical analysis. Prentice-Hall, Englewood Cliffs, New Jersey, pp41–235
Brodie BB, Costa E, Dlabac A, Neff NH, Smookler HH (1966) Application of steady state kinetics to the estimation of synthesis rate and turnover time of tissue catecholamine. J Pharmacol Exp Ther 154: 493–498
Neff NH, Tozer TN, Hammer W, Costa E, Brodie BB (1968) Application of steady-state kinetics to the uptake and decline of 3H-NE in the rat heart. J Pharmacol Exp Ther 160: 48–52
Taubin HL, Djahanguiri B, Landsberg L (1972) Noradrenaline concentration and turnover in different regions of the gastrointestinal tract of the rat: an approach to the evaluation of sympathetic activity in the gut. Gut 13: 790–795
Vander Tuig JG, Ohshima K, Yoshida T, Romsos DR, Bray GA (1984) Adrenalectomy increases norepinephrine turnover in brown adipose tissue of obese (ob/ob) mice. Life Sci 34: 1423–1432
Seydoux J, Chinet A, Schneider-Picard G, Bas S, Imesch E, Assimacopoulos-Jeannet F, Giacobino JP, Girardier L (1983) Brown adipose tissue metabolism in streptozotocin diabetic rats. Endocrinology 113: 604–610
Rothwell NJ, Stock MJ (1981) A role for insulin in diet-induced thermogenesis of cafeteria-fed rats. Metabolism 30: 673–678
Giachetti A (1978) The functional state of sympathetic nerves in spontaneously diabetic mice. Diabetes 27: 969–974
Young JB, Einhorn D, Landsberg L (1983) Decreased sympathetic (SNS) activity in interscapular brown adipose tissue (IBAT) of streptozotocin-treated rats. Diabetes 32 (Suppl 1): 26 A (Abstract)
Nadeau A, Tancrede G, Trudel D, Jobidon C, Nguyen MH, Rousseau-Migneron S (1983) Decreased sympathetic activity in the pancreas of streptozotocin-diabetic rats. Diabetes 32 (Suppl 1): 146 A (Abstract)
Yoshida T, Bray GA (1984) Catecholamine turnover in rats with ventromedial hypothalamic lesions. Am J Physiol 246: R558-R565
Young JB, Saville E, Rothwell NJ, Stock MJ, Landsberg L (1982) Effect of diet and cold exposure on norepinephrine turnover in brown adipose tissue of the rat. J Clin Invest 69: 1061–1071
Hernandez L, Briese E (1979) Analysis of diabetic hyperphagia and polydipsia. Physiol Behav 9: 741–746
De Castro JM, Balagura S (1975) Meal patterning in the streptozotocin-diabetic rats. Physiol Behav 15: 259–263
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Yoshida, T., Nishioka, H., Nakamura, Y. et al. Reduced noradrenaline turnover in streptozotocin-induced diabetic rats. Diabetologia 28, 692–696 (1985). https://doi.org/10.1007/BF00291978
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DOI: https://doi.org/10.1007/BF00291978