Elsevier

Methods

Volume 11, Issue 3, March 1997, Pages 319-329
Methods

Regular Article
Interplay between NO and [Fe-S] Clusters: Relevance to Biological Systems

https://doi.org/10.1006/meth.1996.0426Get rights and content

Abstract

In mammalian cells, nitric oxide (NO) synthesis results in the inactivation of several mitochondrial iron-sulfur enzymes involved in ATP synthesis that correlates with the appearance of complexes of the [(NO)2Fe(SR)2] type detectable by electron paramagnetic resonance spectroscopy. More specifically, the activity of two [Fe-S] enzymes was followed during the course of NO synthase expression:mitochondrial aconitase, which catalyzes citrate:isocitrate conversion in the Krebs cycle, and cytoplasmic aconitase, or iron regulatory protein (IRP), atrans-regulator that controls expression at the posttranscriptional level of proteins involved in iron metabolism. In response to physiological stimuli, the synthesis of NO leads to inhibition of enzymatic activities of both mitochondrial and cytoplasmic aconitases, whereas the RNA binding activity of IRP is increased. Coordination of the diffusible gas NO with [Fe-S] clusters is thought to result in impairment of metabolic functions. Here it is proposed that the interplay between NO (or some NO-derived molecule) and [Fe-S] clusters at critical catalytic or allosteric sites is crucial in the response to environmental signals within cells.

References (113)

  • J.B. Hibbs et al.

    Biochem. Biophys. Res. Commun.

    (1988)
  • C. Nathan et al.

    Cell

    (1994)
  • P.C. Ford et al.

    FEBS Lett.

    (1993)
  • D. Servent et al.

    Biochim. Biophys. Acta

    (1991)
  • D.A. Wink et al.

    Arch. Biochem. Biophys.

    (1993)
  • D.L. Rousseau et al.

    J. Biol. Chem.

    (1988)
  • M.J. Nelson

    J. Biol. Chem.

    (1987)
  • J.C. Woolum et al.

    Biochim. Biophys. Acta

    (1970)
  • A.R. Butler et al.

    Polyhedron

    (1985)
  • A. Mülsch et al.

    Biochem. Biophys. Res. Commun.

    (1993)
  • A.R. Butler et al.

    Inorg. Chim. Acta

    (1985)
  • J. Meyer

    Arch. Biochem. Biophys.

    (1981)
  • M.E. Murphy et al.
  • C. Pellat et al.

    Biochem. Biophys. Res. Commun.

    (1990)
  • J.C. Drapier et al.

    J. Biol. Chem.

    (1991)
  • N. Welsh et al.

    Biochem. Biophys. Res. Commun.

    (1992)
  • J.R. Lancaster et al.

    J. Biol. Chem.

    (1992)
  • J.A. Corbett et al.

    J. Biol. Chem.

    (1991)
  • N. Bastian et al.

    J. Biol. Chem.

    (1994)
  • Y.J. Geng et al.

    Exp. Cell Res.

    (1994)
  • J. Stadler et al.

    Arch. Biochem. Biophys.

    (1993)
  • D.W. Reif et al.

    Arch. Biochem. Biophys.

    (1990)
  • A.F. Vanin et al.

    Biochim. Biophys. Acta

    (1992)
  • M.C. Kennedy et al.

    Biochem. Biophys. Res. Commun.

    (1993)
  • T.A. Rouault et al.

    Cell

    (1991)
  • R.D. Klausner et al.

    Cell

    (1993)
  • C.C. Philpott et al.

    J. Biol. Chem.

    (1993)
  • E.W. Müllner et al.

    Cell

    (1989)
  • B. Guo et al.

    J. Biol. Chem.

    (1994)
  • F. Samaniego et al.

    J. Biol. Chem.

    (1994)
  • R. Oria et al.

    Blood

    (1995)
  • K. Pantopoulos et al.

    Trends Cell Biol.

    (1994)
  • J.C. Drapier et al.

    J. Inorg. Chem.

    (1994)
  • R.S. Eisenstein et al.

    Proc. Natl. Acad. Sci. USA

    (1993)
  • A. Hausladen et al.

    J. Biol. Chem.

    (1994)
  • L. Castro et al.

    J. Biol. Chem.

    (1994)
  • R. Radi et al.

    Arch. Biochem. Biophys.

    (1994)
  • D.L. Granger et al.

    J. Clin. Invest.

    (1980)
  • J.B. Hibbs et al.

    J. Immunol.

    (1987)
  • J.C. Drapier et al.

    J. Immunol.

    (1988)
  • D.L. Granger et al.

    J. Cell Biol.

    (1982)
  • Y. Hatefi

    Annu. Rev. Biochem.

    (1985)
  • J.C. Drapier et al.

    J. Clin. Invest.

    (1986)
  • J.B. Hibbs et al.

    Science

    (1987)
  • D.J. Stuehr et al.

    Proc. Natl. Acad. Sci. USA

    (1985)
  • D.J. Stuehr et al.

    J. Immunol.

    (1987)
  • R.M.J. Palmer et al.

    Nature

    (1992)
  • L.J. Ignarro et al.

    Proc. Natl. Acad. Sci. USA

    (1987)
  • R.M.J. Palmer et al.

    Nature

    (1988)
  • M.A. Marletta et al.

    Biochemistry

    (1988)
  • Cited by (115)

    View all citing articles on Scopus

    L. Packer, Ed.

    View full text