Regular ArticleOntogeny of Cardiacβ-Adrenoceptor Desensitization Mechanisms: Agonist Treatment Enhances Receptor/G-Protein Transduction Rather than Eliciting Uncoupling
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Cardiac Physiology and Pharmacology
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2011, Pediatric Critical CareOrganophosphate exposure during a critical developmental stage reprograms adenylyl cyclase signaling in PC12 cells
2010, Brain ResearchCitation Excerpt :Our AC assessments focused on measures that evaluate pathway function at sequential steps: basal enzymatic activity, the response to global stimulation of G-proteins by fluoride, and the responses to two direct AC stimulants, forskolin and Mn2+. Because the two stimulants act at different epitopes on the AC molecule, the preferential effects for one versus the other define shifts in the expression and catalytic activities of different AC isoforms (Auman et al., 2000; Zeiders et al., 1997, 1999a). We then compared the effects on AC signaling to those on cell growth, focusing on measures of cell number and neurite formation.
Neonatal parathion exposure and interactions with a high-fat diet in adulthood: Adenylyl cyclase-mediated cell signaling in heart, liver and cerebellum
2010, Brain Research BulletinCitation Excerpt :In addition to assessing basal AC activity, we evaluated responses to 100 μM isoproterenol, 3 μM glucagon, 10 mM NaF and 100 μM forskolin. These concentrations produce maximal responses to each stimulant as assessed in earlier studies [3,31,32]. To evaluate βAR binding, aliquots of the same membrane preparation were incubated with 67 pM [125I]-iodopindolol in 145 mM NaCl, 2 mM MgCl2, 1 mM sodium ascorbate, 20 mM Tris (pH 7.5), for 20 min at room temperature; samples were evaluated with and without 100 μM isoproterenol to displace specific binding.
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Please address correspondence to: Dr T. A. Slotkin, Box 3813, Department of Pharmacology & Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.