Elsevier

Genomics

Volume 37, Issue 3, 1 November 1996, Pages 390-394
Genomics

Short Communication
Cloning, RNA Expression, and Chromosomal Location of a Mouse Histamine H2 Receptor Gene

https://doi.org/10.1006/geno.1996.0575Get rights and content

Abstract

A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase–polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designatedHrh2,was located in the central region of chromosome 13.

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The nucleotide sequence data from this article have been deposited with the DDBJ, EMBL, and GenBank Nucleotide Sequence Data Libraries under Accession No. D50096. The chromosome mapping data have been deposited with the MGD Database under Accession No. MGD-CREX-673.

1

Present address: Laboratory for Bone Research, Research and Development Division, Nippon Hoechst Marion Roussel, Ltd., 1-3-2 Minamidai, Kawagoe, Saitama 350-11, Japan.

2

To whom correspondence should be addressed. Telephone: 81-92-641-1151 (ext. 3781). Fax: 81-92-632-1499.

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