Brief CommunicationImmortalized GABAergic Cell Lines Derived from Rat Striatum Using a Temperature-Sensitive Allele of the SV40 Large T Antigen
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Lack of effect of intranigral transplants of a GABAergic cell line on absence seizures
2010, Epilepsy and BehaviorCitation Excerpt :Intranigral transplants of M213-2O CL-4 cells significantly increased GABA content in the area of the transplant [15]. This cell line was obtained after transfection of cell line M231-20, a cell line immortalized using the tsA58 of the SV40 large T antigen, which expresses GAD constitutively [18,19], with the human glutamate decarboxylase-67 cDNA using an episomal plasmid vector based on the Epstein–Barr virus [20]. Given the previous results obtained in other animal models, in the present study the GAERS model of chronic epilepsy was used to test, over a 5-month period, if intranigral cell transplants of this GABAergic cell line have functional effects in a genetic model that shares the condition of chronically recurrent absence seizures in human patients [6].
Intranigral transplants of a GABAergic cell line produce long-term alleviation of established motor seizures
2008, Behavioural Brain ResearchBenefits and risks of intranigral transplantation of GABA-producing cells subsequent to the establishment of kindling-induced seizures
2008, Neurobiology of DiseaseCitation Excerpt :Instead, the anticonvulsant effect we observed after intranigral transplantation of M213-2O cells in kindled rats was most likely attributed to an increased GABAergic inhibition of the SNr, most likely due to tonic, non-synaptic release of GABA. This is supported by studies, which showed that M213-2O cells produce GABA in vitro (Conejero-Goldberg et al., 2000; Giordano et al., 1993). However, we have no direct evidence that the effects of M213-2O cell grafts on seizures were linked to increased GABA levels in the SNr.
Establishment of an immortalized GABAergic neuronal progenitor cell line from embryonic ventral mesencephalon in the rat
2008, Brain ResearchCitation Excerpt :It has also been reported that cells pre-differentiated toward a GABAergic phenotype prior to intrastriatal transplantation can replace the population of lost cells and restore the function of damaged striatal circuits in HD (Bosch et al., 2004). Until now, it's still a problem about how to harvest sufficient amounts of GABAergic neurons/precursors for transplantation, although there have already been some reports about the immortalized GABAergic cell lines derived from rat striatum or rat mesencephalon (Giordano et al., 1993; Sanchez et al., 2006). Neuroprotection is another promising therapy for several neurological diseases, since deprivation of neurotrophic support is one of the multiple pathways leading to cell death in those diseases (Ferrer et al., 2000; Howells et al., 2000; Lin et al., 1993; Salehi et al., 2004).