Elsevier

Experimental Neurology

Volume 124, Issue 2, December 1993, Pages 395-400
Experimental Neurology

Brief Communication
Immortalized GABAergic Cell Lines Derived from Rat Striatum Using a Temperature-Sensitive Allele of the SV40 Large T Antigen

https://doi.org/10.1006/exnr.1993.1213Get rights and content

Abstract

Embryonic striatal cells were immortalized using the A58 temperature-sensitive allele of the SV40 large T antigen. Two cell lines, M213-2O and M26-1F, with γ-aminobutyric acid (GABA)ergic properties were selected from 85 clones thus developed. M213-20 is a multipolar, polygonal cell line which expresses SV40 large T antigen and glutamate decarboxylase (GAD) at the permissive temperature (33°C) and GAD and MAP-2 immunoreactivity at the nonpermissive temperature (39.3°C). M26-1F has a fibroblast-like morphology and expresses SV40 large T antigen and GAD both at the permissive and nonpermissive temperatures and MAP-2 immunoreactivity at the nonpermissive temperature. Both lines contain GABA as measured by reversed-phase HPLC and M213-2O expresses nipecotic-sensitive [14C]GABA uptake.

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