MODULATION OF IL-1-INDUCED CHEMOKINE EXPRESSION IN HUMAN MESANGIAL CELLS THROUGH ALTERATIONS IN REDOX STATUS

doi:10.1006/cyto.1996.0152

Cytokine

Volume 9, Issue 3, March 1997, Pages 178-186

https://doi.org/10.1006/cyto.1996.0152 Get rights and content

Abstract

The effects of altering intracellular redox potential on interleukin 1 (IL-1)-induced MCP-1 gene expression by human mesangial cells were examined. Thiol containing antioxidants significantly increased cellular glutathione content while decreasing glutathione disulfide levels. These antioxidants inhibited IL-1 induction of MCP-1 mRNA expression. This correlated with a decrease in DNA binding activity of NFκB, a transcription factor thought to be necessary for MCP-1 gene expression Incubation of mesangial cells with the oxidizing agents diamide or hydrogen peroxide did not upregulate MCP-1 gene expression, and prevented IL-1 induction of MCP-1 mRNA. Oxidants appeared to inhibit the degradation of IκB, and the translocation of NF-κB to the nucleus. Non-oxidative depletion of intracellular glutathione also attenuated the effects of IL-1 on MCP-1 expression. These data indicate that the intracellular redox potential is a critical determinant of cell activation by IL-1. The observation that both oxidizing and reducing environments are inhibitory suggests that redox changes can affect the IL-1 signal tranduction pathway at multiple points.

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^f1: Correspondence to: Brad H. Rovin, Nephrology Division, Ohio State University, 1654 Upham Dr., Columbus, OH 43210, USA

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Regular articleMODULATION OF IL-1-INDUCED CHEMOKINE EXPRESSION IN HUMAN MESANGIAL CELLS THROUGH ALTERATIONS IN REDOX STATUS

Abstract

Regular article
MODULATION OF IL-1-INDUCED CHEMOKINE EXPRESSION IN HUMAN MESANGIAL CELLS THROUGH ALTERATIONS IN REDOX STATUS