Regular ArticleQuantification of Platelet Activation Status by Analyzing P-Selectin Expression
References (20)
Flow cytometry: A clinical test of platelet function
Blood
(1996)- et al.
Platelet surface P-selectin measurements in platelet preparations: An international collaborative study
Transfus. Med. Rev.
(1999) - et al.
Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry
Blood
(1987) - et al.
Flow cytometric parameters for characterizing platelet activation by measuring P-selectin (CD62) expression: Theoretical consideration and evaluation in thrombin-treated platelet populations
Biochem. Biophys. Res. Commun.
(2000) - et al.
Flow cytometric analysis of the platelet surface area and surface density of glycoprotein IIb-IIIa of unactivated human platelets of various sizes
Biochem. Biophys. Res. Commun.
(1996) - et al.
Flow cytometric analysis of platelet function in stored platelet concentrates
Transfus. Sci.
(1999) - et al.
Downregulation of the platelet surface glycoprotein Ib-IX complex in whole blood stimulated by thrombin, adenosine diphosphate, or an in vivo wound
Blood
(1991) - et al.
Activation in stored platelet concentrates: correlation between membrane expression of P-selectin, glycoprotein IIb/IIIa, and β-thromboglobulin release
Transfusion
(1993) - et al.
Circulating platelets show increased activation in patients with acute cerebral ischemia
Thromb. Haemost.
(1999)
Cited by (34)
Cyclic RGD functionalized liposomes encapsulating urokinase for thrombolysis
2018, Acta BiomaterialiaCitation Excerpt :In our study, the platelets collected by differential centrifugation from whole blood of volunteer human were referred as freshly prepared platelets. The expression level of P-selectin (PE-anti-CD62P) on membranes was determined as a biomarker of the activation status of platelets [9,21,22]. The flow cytometric results demonstrated that cRGD liposomes had slight interaction with the freshly prepared platelets (Fig. 3A), which could attribute to the part and slight activation of the freshly prepared platelets (Supplementary Fig. 5B and C).
New peptide pENW (pGlu-Asn-Trp) inhibits platelet activation by attenuating Akt phosphorylation
2012, European Journal of Pharmaceutical SciencesCitation Excerpt :As platelet aggregation is induced by various agonists and amplified by active substances secreted from granules, like ADP, serotonin and calcium (Rivera et al., 2009), that pENW significantly inhibited platelet granules secretion induced by agonists would be expected to contribute further to its antiplatelet effect (Fig. 4). Exposure of P-selectin, a content of platelet α-granule, is a marker for platelet activation (Leytin et al., 2000). It stabilizes platelet aggregation along with initial αIIbβ3–fibrinogen interactions (Merten and Thiagarajan, 2000) as well as promotes platelet–leukocyte aggregates by interacting with P-selectin glycoprotein-1 (PSGP-1) on leukocytes (Evangelista et al., 2007).
Development of an immunopredictor for the evaluation of the risk of cardiovascular diseases based on the level of soluble P-selectin
2012, MethodsCitation Excerpt :The key element of constructing an immunosensor relies on antibodies that react specifically with the antigens of interest, with quantitation commonly achieved by measuring a specific activity of the label, e.g., its enzyme amplification [10], radioactivity [11], electrochemical signal [12–15], fluorescence [16], or bioluminescence [17]. Although several methods for the detection of P-selectin have been described based on original or improved assay systems of the flow cytometry [5,18], such as Western blot analysis [19–21] or enzyme-based immunoassay [22], these techniques often require complicated and time-consuming sample pretreatment or sophisticated instrumentation. Herein we present a simple yet novel electrochemical immunosensor featuring anti-P-selectin-tagged, potassium ferrocyanide-encapsulated liposomes as bioprobes for the trace determination of P-selectin at picogram per milliliter levels.
Exaggerated platelet reactivity to physiological agonists in war veterans with posttraumatic stress disorder
2011, PsychoneuroendocrinologyCitation Excerpt :Additionally, mean fluorescence intensity (m.f.i.) of fluorescence channel 2 (PE emission signal), representing CD62P expression, was recorded. The percentage of CD62P-positive platelets is better suited for revealing the differences between populations in the area of low platelet activation while m.f.i. better distinguishes the differences in the area of high activation (Leytin et al., 2000). In the second run, the cytometer was set for analysis of PLA.
Pathologic high shear stress induces apoptosis events in human platelets
2004, Biochemical and Biophysical Research Communications
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To whom correspondence should be addressed at the Department of Transfusion Medicine, Shuter Room 2003, St. Michael's Hospital, 30 Bond Street, Toronto, Ontario M5B 1W8, Canada. Fax: +1 416 864 5294. E-mail: [email protected].