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Structure and Developmental Expression of the Mouse CCK-B Receptor Gene

https://doi.org/10.1006/bbrc.2000.2875Get rights and content

Abstract

Cholecystokinin (CCK) and gastrin exert their effects through two receptors, the CCK-A and CCK-B receptors. We have cloned the mouse CCK-B receptor gene (Cckbr) and determined its complete genomic structure, nucleotide sequence, and tissue-specific expression pattern. Cckbr is divided into five exons spanning 11 kb. A primer extension assay was used to map the transcription initiation site to 199 bp upstream of the translational start site. Rapid amplification of cDNA ends was used to localize the 3′ end downstream of an atypical polyadenylation site (GATAAA). Mouse Cckbr transcripts were most abundant in brain and stomach, but were also detected in colon, kidney, ovary, and pancreas. Prenatal expression of both CCK-A and CCK-B receptors in various tissues was analyzed by RT-PCR. The expression pattern was similar to the adult pattern, suggesting that receptor transcription is an early event in gastrointestinal development.

References (36)

  • J.R. Pisegna et al.

    Molecular cloning of the human brain and gastric cholecystokinin receptor: Structure, functional expression and chromosomal localization

    Biochem. Biophys. Res. Commun.

    (1992)
  • M. Wickens

    How the messenger got its tail: Addition of poly(A) in the nucleus

    Trends Biochem. Sci.

    (1990)
  • P. Epstein et al.

    Isolation of a rat parvalbumin gene and full length cDNA

    J. Biol. Chem.

    (1986)
  • S. Nomoto et al.

    Cloning and characterization of the alternative promoter regions of the human LIMK2 gene responsible for alternative transcripts with tissue-specific expression

    Gene

    (1999)
  • J.C. Jean et al.

    The Bax inhibitor-1 gene is differentially regulated in adult testis and developing lung by two alternative TATA-less promoters

    Genomics

    (1999)
  • S.A. Wank

    G protein-coupled receptors in gastrointestinal physiology. I. CCK receptors: An exemplary family

    Am. J. Physiol.

    (1998)
  • S.A. Wank et al.

    Purification, molecular cloning, and functional expression of the cholecystokinin receptor from rat pancreas

    Proc. Natl. Acad. Sci. USA

    (1992)
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    1

    Current address: Department of Clinical Biochemistry, Rigshospitalet, Blegdamsvej, DK-2100 Copenhagen, Denmark.

    2

    To whom correspondence should be addressed at Department of Physiology, University of Michigan, Ann Arbor, MI 48109-0622. Fax: (734)936-8813. E-mail: [email protected].

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