Research Articles
High-pressure liquid chromatographic determination of ftorafur [1-(tetrahydro-2-furanyl)-5-fluorouracil] and GLC-mass spectrometric determination of 5-fluorouracil and uracil in biological materials after oral administration of uracil plus ftorafur

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Abstract

Oral adminstration of uracil plus ftorafur [1-(tetrahydro-2-furanyl)-5-fluorouracil] has a greater antitumor effect than that of ftorafur alone. A high-pressure liquid chromatographic method was developed for the determination of ftorafur in plasma and visceral tissues with a sensitivity of 0.025 μg/ml or g of wet weight. A combination of GLC-mass fragmentography and GLC-mass spectrometry with total-ionmonitoring was developed for the specific, simultaneous determination of 5-fluorouracil, an active metabolite, and uracil as their trimethylsilylated derivatives. The detection limits for 5-fluorouracil and uracil in the first method were 0.001 μg/ml for plasma and 0.001-0.005 μg/ml for visceral tissues, and those in the second method were 0.2 μg/ml or g of wet weight. The precision and sensitivity of the assay appear to be satisfactory for determination of the levels of these compounds in plasma and visceral tissues.

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