Relaxation of precontracted (with 1 μM PE) HCC strips from control subjects and from patients with vasculogenic ED to 1 to 10 μM ACh: the role of NO production The NO synthase inhibitor l-NOARG (100 μM) was added to the incubation media 15 min before application of ACh. Values are expressed as percentage ± S.E.M. of maximal contraction caused by 1 μM PE. The number of separated human specimens (four strips for each experiment) is shown in parentheses.
Control Individuals | Patients with Vasculogenic ED | ||
|---|---|---|---|
| % | |||
| No l-NOARG (100 μM) | |||
| + ACh (1 μM) | 53 ± 8 (14) | 81 ± 6 (8)* | |
| + ACh (10 μM) | 43 ± 6 (13) | 67 ± 5 (8)* | |
| With l-NOARG (100 μM) | |||
| + ACh (1 μM) | 92 ± 2 (5)* | N.T. | |
| + ACh (10 μM) | 77 ± 6 (5)* | N.T. | |
N.T., not tested
↵* P < 0.05 compared with the effect of ACh in control subjects in the absence of l-NOARG