Apparent Michaelis-Menten kinetics and specificity constant for recombinant rat Nat1, Nat2, and Nat3
Apparent Vmax | Apparent Km | Specificity Constant | |
|---|---|---|---|
| nmol/min/mg | μM | nmol/min/mg/μM | |
| AF | |||
| Nat1 | 27.8 ± 1.9 | 37.0 ± 4.2 | 0.751 |
| Nat2 | 644 ± 24 | 568 ± 44 | 1.13 |
| Nat3 | 6.52 ± 0.08 | 33.1 ± 1.0 | 0.197 |
| ABP | |||
| Nat1 | 34.0 ± 1.8 | 5.83 ± 1.05 | 5.83 |
| Nat2 | 706 ± 50 | 1570 ± 213 | 0.448 |
| Nat3 | 4.33 ± 0.10 | 18.2 ± 1.6 | 0.238 |
| N-OH-ABP | |||
| Nat1 | 0.633 ± 0.014 | 58.5 ± 2.0 | 0.0108 |
| Nat2 | 2.03 ± 0.10 | 3300 ± 380 | 0.000615 |
| Nat3 | 0.162 ± 0.007 | 62.0 ± 7.5 | 0.00262 |