Summary of basal and stimulated parameters in WT, Y4-/-, or Y1-/- mouse isolated colon mucosae, human colon mucosa, or HT-29 epithelia transfected with hY4 receptors Transepithelial resistances, basal Isc, the peak VIP responses, and their subsequent inhibition by a maximal PYY or hPP concentration (HT-29 cells only) are indicated as mean ± 1 S.E.M. with n values given in parentheses.
Preparation | Basal Resistance | Basal Isc | VIP Isc [30 nM] | |
|---|---|---|---|---|
| Ω · cm2 | μA/cm2 | |||
| WT mouse mucosa | 33.8 ± 1.3 (187) | 46.8 ± 2.2 (187) | 154.0 ± 6.7 (187) | |
| Y4-/- mucosa | 33.5 ± 1.4 (155) | 46.0 ± 2.5 (155) | 184.8 ± 7.7 (155)* | |
| Y1-/- mucosa | 24.1 ± 1.6 (79)* | 76.4 ± 5.7 (79)* | 117.2 ± 8.4 (79)* | |
| Human mucosa | 99.0 ± 7.4 (19) | 97.2 ± 12.4 (19) | ||
| HT-29 hY4 layers | 20.6 ± 2.9 (26) | 15.6 ± 1.7 (26) | 25.1 ± 3.2 (26) | |
↵* P < 0.01 compared with WT mucosa using one-way ANOVA with Dunnett's post-test