TABLE 4

Effect of plant cannabinoids on anandamide inactivation

Membranes from N18TG2 cells were incubated with [14C]anandamide in the presence of compounds for 30 min at 37°C (see Material and Methods) to determine the effect on the enzymatic hydrolysis by fatty acid amide hydrolase (FAAH). Intact RBL-2H3 cells were incubated with [14C]anandamide in the presence of compounds for 5 min at 37°C (see Material and Methods) to determine the effect on anandamide cellular uptake (ACU). Data represent mean ± S.E. of three different experiments.




FAAH Assay IC50

ACU Assay IC50
μM
THC >50 22 ± 5
CBD 28 ± 3a 22 ± 2a
CBG >50 15 ± 3
CBC >50 13 ± 2
THC-A >50 >25
CBD-A
>50
>25
  • CBG, cannabigerol; CBC, cannabichromene; CBD-A, cannabidiol-acid; THC-A, THC-acid.

  • a Data from Bisogno et al., 2001.