Composition of solutions used for the skinned fiber experiments (pCa = −log10[Ca2+])
Solution and Function | Parameter | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| pCa | [EGTA] | [Mg2+] | [Caffeine] | Incubation Time | |||||
| mM | min | ||||||||
| Basic solutions | |||||||||
| Relaxing | 9.0 | 10 | 1 | 0 | |||||
| Maximal activating | 4.5 | 10 | 1 | 0 | |||||
| Saponin experiment solutions | |||||||||
| 1. Loading | 7.0 | 10 | 1 | 0 | 2 | ||||
| 2. Washing | 7.5 | 0.4 | 0.1 | 0 | 2 | ||||
| 3. Releasing | 7.5 | 0.4 | 0.1 | 10 | Contracture duration | ||||