Conditions for autoradiography on sagittal sections from rat brain Radioligands were from PerkinElmer Life and Analytical Sciences (Boston, MA). Film was BioMax MR-2 film (35 × 43 cm; no. 865 7232; Eastman Kodak, Rochester, NY). Films were manually processed using Kodak GBX developer and fixer according to the manufacturer's protocol.
Ligand | Epibatidine | α-Bungarotoxin | Pirenzepine | AFDX-384 |
|---|---|---|---|---|
| Receptor subtype target | Nicotinic non-α7 | Nicotinic α7 | Muscarinic M1/M4 | Muscarinic M2 |
| Catalog no. | NET-1102 | NEX-1265 | NET-780 | NET-1041 |
| Isotope | 3H | 125I | 3H | 3H |
| Specific activity | 55.5 Ci/mmol | 17.0 μCi/μg | 86 Ci/mmol | 120 Ci/mmol |
| Radioligand | ||||
| Incubation | 0.8 | 1.0 | 3.0 | 1.8 |
| Concentration (nM) | ||||
| Preincubation time in buffer (min) | None | 20 | 15 | 15 |
| Radioligand incubation time (min) | 40 | 120 | 90 | 90 |
| Film exposure time | 13 wk | 160 h | 6 wk | 16 wk |