Primer sequences used for quantitative real-time PCR Sequences are listed 5′ to 3′ end. The primers are named according to the receptor assessed.
β-Actinfw | CCGGGACCTGACAGACTA |
| β-Actinrev | GTTTCATGGATGCCACAGGAT |
| hmLPA1fw | CTGAAGACTGTGGTCATTGTGC |
| hmLPA1rev | AACCACAGAGTGGTCATTGCTG |
| hLPA2fw | GTCAAGACTGTTGTCATCATCCT |
| hLPA2rev | GGAAGCATGATGCGAGTGCG |
| mLPA2fw | GTCAAGACGGTTGTCATCATTCT |
| mLPA2rev | GAAGCATGATCCGCGTGCT |
| mLPA3fw | ACAAAGCTTGTGATCGTCCTGT |
| mLPA3rev | TCATGATGGACATGTGTCTTTCC |
| hLPA3fw | GATTGTTTTGTGTGTTGGGACG |
| hLPA3rev | TGGTCAGGTTGCTATGGACC |
| hGPR23fw | CACCAATCTAGCTGTCTCTGATT |
| hmGPR23rev | GCATTGTTGACATTAGTGGTGGA |
| mGPR23fw | AACCTGGCCCTCTCTGATTT |
| hGPR92fw | CTCTCCTACGCACTGCACCACT |
| hGPR92rev | GAAGCTCTCGAAGCATAGGCGCA |
| mGPR92fw | CCGTACATGTTCATCTGGAAGAT |
| mGPR92rev | CAGACTAATTTCTCTTCCCACCT |
m, mouse; h, human; fw, forward; rev, reverse primers