Levels of AL1576 in the soluble fraction of human myocardial strips exposed to doxorubicin and taxane-vehicle formulations Human myocardial strips were incubated in plasma and exposed to 10 μM doxorubicin, 6 μM taxanes formulated in their cosolvent systems (cf. legends to Tables 2, 3, 4), and 1 to 500 μM AL1576. After 4 h, the soluble fraction of the strips was assayed for AL1576 as described under Materials and Methods. All of the values were means ± S.E. of three experiments and were analyzed by one-way analysis of variance/Bonferroni's test for multiple comparisons; where indicated, unpaired Student's t test was also applied.
AL1576 in Plasma | AL1576 in the Soluble Fraction | ||
|---|---|---|---|
| Doxorubicin/Paclitaxel-Vehicle Strips | Doxorubicin/Docetaxel-Vehicle Strips | ||
| μM | μM | ||
| 1 | 0.2 ± 0.04 | 0.2 ± 0.06 | |
| 10 | 2.7 ± 0.2a | 3.5 ± 0.7b | |
| 100 | 27.9 ± 3.3c | 57.7 ± 5.8d | |
| 500 | 187.3 ± 24e | 277.3 ± 52f | |
↵ a p > 0.05 for 10 versus 1 μM (p < 0.001 by unpaired Student's t test)
↵ b p > 0.05 for 10 versus 1 μM (p < 0.001 by unpaired Student's t test)
↵ c p > 0.05 for 100 versus 10 μM (p < 0.001 by unpaired Student's t test)
↵ d p > 0.05 for 100 versus 10 μM (p < 0.001 by unpaired Student's t test)
↵ e p < 0.01 for 500 versus 1–100 μM
↵ f p < 0.01 for 500 versus 1–100 μM