Binding affinities of thienorphine for rat μ-, δ-, and κ-opioid receptors stably expressed in CHO cells Competitive binding assays were performed as described under Materials and Methods. Ki values with 95% confidence limits were calculated by nonlinear regression using Prism 4.0.
Ki (95% Confidence Limits) | |||||
|---|---|---|---|---|---|
| μ | δ | κ | |||
| nM | |||||
| Thienorphine | 0.10 (0.06–0.17) | 0.27 (0.16–0.46) | 0.23 (0.10–0.49) | ||
| Buprenorphine | 0.51 (0.25–1.03) | 0.91 (0.49–1.70) | 0.21 (0.14–0.31) | ||
| Morphine | 24.80 (15.26–40.31) | 213.9 (135.8–337.0) | 133.1 (96.88–182.7) | ||