TABLE 4

Endorem uptake by rodent brain-derived EC219 endothelial cells or murine N11 microglial cells Rat brain-derived EC219 endothelial cells were exposed to Endorem [0.4 mM iron equivalents added at t = 0 simultaneously with (+) LPS or without (-) LPS], or murine N11 microglial cells were exposed to Endorem [1.0 mM iron equivalents added at t = 0 simultaneously with (+) LPS or without (-) LPS] for 16 or 24 h. Then cell-bound iron was measured using the Prussian Blue reaction, and means ± S.D. were calculated. Experiments were repeated twice in triplicate wells.



EC 219

N11

–LPS
+LPS
–LPS
+LPS
Time
Cell-Bound Iron
h μM
16 54 ± 11 53 ± 10 102 ± 19 166 ± 24
24
62 ± 15
54 ± 12
122 ± 19
214 ± 32