Effects of Tat on the [3H]NE release evoked by NMDA/glycine from rat cortical noradrenergic nerve endings: effects of I group mGluR antagonists
Synaptosomes were superfused with Mg2+-free medium; rat synaptosomes were exposed to NMDA (100 μM) + glycine (1 μM). Tat (final concentration 1 nM) was added concomitantly with NMDA/glycine. Group I mGluR antagonists MPEP (1 μM) and LY 367385 (1 μM) were added 8 min before agonists until the end of the superfusion period. Results are expressed as percentage increase over basal release; data are means ± S.E.M. of three experiments run in triplicate.
NMDA + Glycine-Induced [3H]NE Release Percentage of Increase | |
|---|---|
| Control | 33.50 ± 3.20 |
| +Tat | 55.00 ± 3.24a |
| +Tat + LY 367385 | 28.86 ± 3.22a |
| +Tat + MPEP | 47.83 ± 2.33 |
↵ a p < 0.001 vs. respective control.