TABLE 2

Arcaine does not affect the release of [³H]NE evoked by NMDA/glycine in absence or in presence of Tat from human and rat nerve endings

Human and rat synaptosomes were superfused with Mg²⁺-free medium and exposed, respectively, to 1 mM NMDA + 3 μM glycine (human synaptosomes) or to 100 μM NMDA + 1 μM glycine (rat synaptosomes). When indicated, 1 nM Tat was added together with agonists, whereas arcaine was present from 8 min before agonists. Results are expressed as percentage increase over basal release; data are means ± S.E.M. of five (human) and three to six (rat) experiments run in triplicate. Arcaine did not affect on its own the spontaneous release of tritium (not shown).