TABLE 2

Effect of cpt-cAMP 0.3 mM on sterol synthesis in J774 murine macrophages and MPMs

Cells were incubated with [2-14C]acetate for 20 h in RPMI 1640 + 0.2% BSA in the presence or absence of 10 μM pitavastatin; 0.3 mM cpt-cAMP was added during the 20-h incubation period. Data are means ± S.D. expressed as counts per minute of [2-14C]acetate incorporation into total cellular sterols per milligram of protein (n = 3).




J774

MPMs
cpm/mg protein
Control 30,042 ± 4260 26,021 ± 5950
cpt-cAMP 15,757 ± 2365** 18,257 ± 4111 (N.S.)
Pitavastatin, 10 μM 4236 ± 305*** 9583 ± 743**
cpt-cAMP + pitavastatin, 10 μM
3000 ± 550
7436 ± 658
  • N.S., not significant versus control.

  • ** p < 0.01; *** p < 0.001 compared with control.

  • p < 0.05 compared with 10 μM pitavastatin.