pEC50 and Emax values derived from concentration-response curves to the sGC stimulators BAY 41-2272 (0.0001–1 μM) and YC-1 (0.001–30 μM) in endothelium-intact mesenteric artery rings contracted with phenylephrine (1 μM)
Curves were performed in the absence and in the presence of the Akt inhibitor (10 μM; n = 4) or the PKG inhibitors KT 5823 (1 μM; n = 4), Rp-8-pCPT-cGMPS (30 μM; n = 4), and DT-3 (1 μM; n = 4). Data represent the means ± S.E.M. of n experiments.
BAY 41-2272 | YC-1 | |||||
|---|---|---|---|---|---|---|
| pEC50 | Emax | pEC50 | Emax | |||
| Control | 8.20 ± 0.06 | 95 ± 3 | 6.70 ± 0.04 | 95 ± 3 | ||
| + Akt inhibitor | 8.11 ± 0.05 | 93 ± 2 | 6.61 ± 0.05 | 91 ± 4 | ||
| Control | 8.08 ± 0.04 | 91 ± 5 | 6.79 ± 0.06 | 93 ± 4 | ||
| + KT 5823 | 8.20 ± 0.04 | 91 ± 6 | 6.68 ± 0.06 | 97 ± 2 | ||
| Control | 8.28 ± 0.05 | 91 ± 8 | 6.64 ± 0.08 | 97 ± 2 | ||
| + Rp-8-pCPT-cGMPS | 8.14 ± 0.05 | 86 ± 7 | 6.58 ± 0.07 | 93 ± 3 | ||
| Control | 8.20 ± 0.06 | 95 ± 3 | 6.73 ± 0.05 | 94 ± 3 | ||
| + DT-3 | 8.06 ± 0.07 | 92 ± 5 | 6.59 ± 0.05 | 91 ± 4 | ||