Anti-HER2/neu antibodies and repair of mechanical wounding
Injured epithelial cells were treated with 100 nM concentrations of anti-HER2/neu antibodies for the indicated times. The injured epithelial monolayers were photographed using a phase-contrast inverted microscope with a 3CCD camera. The width of each wound was measured at three sites in each image, and the percentage of wound recovery was calculated by comparison with the initial wound width. The percentage shown in the column is the mean of the wound recovery from six independent images for each treatment at each time point. The numbers in the parentheses correspond to the mean ± S.D. of the measurement.
| CHO Medium | Anti-HER2/neu ScFv-TNF-α | Anti-HER2/neu ScFv-TNF-α(S147Y) | PBS | Anti-HER2 IgG3 (C6MH3-B1) | Anti-HER2 IgG3 (Herceptin) | |
|---|---|---|---|---|---|---|
| % | ||||||
| 0 h | 0 | 0 | 0 | 0 | 0 | 0 |
| 21 h | 40.6 (6.8) | 66.3 (8.7)a | 61.7 (6.7)a | 41.8 (6.6) | 46.0 (6.7) | 43.5 (1.7) |
| 29 h | 62.7 (8.8) | 97.0 (3.7)a | 94.6 (3.1)a | 68.4 (6.2) | 70.3 (6.9) | 69.2 (3.1) |
↵ a P < 0.001 (n = 6) compared with the control of CHO medium or PBS.