Comparison of kinetic parameters for transport of 2-OG and GSH by bacterially expressed, purified, and reconstituted OGC-WT and OGC-C221,224S
Uptake rates of 2-OG and GSH for the WT and double-cysteine mutant carriers were derived from Eadie-Hofstee plots of 1-min uptake rates derived from data shown in Figs. 2 and 3.
| OGC-WT | OGC-C221,224S | ||
|---|---|---|---|
| 2-OG | Km (mM) | 0.18 ± 0.03 | 2.45 ± 0.42 |
| Vmax (nmol/min/mg/protein) | 475 ± 37 | 143 ± 22 | |
| Vmax/Km | 2639 | 58.4 | |
| GSH | Km (mM) | 1.25 ± 0.15 | 1.43 ± 0.22 |
| Vmax (nmol/min/mg/protein) | 375 ± 42 | 70.2 ± 10.5 | |
| Vmax/Km | 300 | 49.1 |