Tissue-specific differences in nucleotide and amino acid sequences of rat OGC
The published nucleotide and amino acid sequences from the heart, brain, and liver of the rat noted are compared with those of the rat kidney that we obtained in our current study. Sequences were aligned using BLAST.
| Tissue/Accession Nos. | Nucleotide Sequences | Amino Acid Sequences |
|---|---|---|
| vs. Heart OGC GenBank accession nos: DNA: X80075; Protein: 2116232A | Identity: 958/964 (99.4%) a28-g48; c129-a149; c145-t165; c227-t247; t228-c248; t600-c620 | Identity: 312/314 (99.4%) T3-A3; T69-I69 |
| vs. Brain OGC GenBank accession nos: DNA: NM_022398, U84727 (source); Protein: AAB41797, NP_071793 | Identity: 934/943 (99.0%) a28-g7; g160-n139; a182-g161; c189-t168; a433-c412; t600-c579; g698-t677; g785-a764; g958-a937 | Identity: 308/314 (98.1%) T3-A3; K46-N46; T138-P138; S226-I226; G255-E255; G313-S313 |
| vs. Liver ODC GenBank accession nos: DNA: AJ289714a Protein: CAC277162 | Identity: no significant homologies | Identity: 72/266 (27.1%) |
↵ a The carrier cDNA with accession number AJ289714 is listed in GenBank as the rat mitochondrial oxodicarboxylate carrier. It is described in the original reference (Fiermonte et al., 2001) as an exchanger that transports 2-OG as well as several other C2-C5 dicarboxylates. mRNA for the carrier was detected by RT-PCR in mitochondria of rat liver, kidney, heart, lung, spleen, testes, skeletal muscle, and brain. The protein was detected by immunodetection in mitochondria of rat heart, kidney, liver, lung, spleen, and brain. The relationship of the ODC with the OGC, however, is unclear.