TABLE 2

Effect of PMN-depleting antiserum on leukocyte counts in blood from LPS/TVX-treated rats

Rats were pretreated with either 0.25 ml of control rabbit serum or rabbit anti-rat PMN serum diluted 1:1 in sterile saline (i.v.). Sixteen hours later, they were given 44.4 × 106 EU/kg LPS or its Veh (i.v.), and then 2 h later, 60 mg/kg TVX or its Veh was administered (i.v.). Total leukocyte and differential counts were performed 6 h after TVX administration as described under Materials and Methods. All values are cells per microliter blood.

Treatment Number of Cells/Microliter Blood
Leukocytes Mature PMNs Monocytes Band Cells Lymphocytes
Control serum/Veh/Veh 7544 ± 430 1238 ± 183 249 ± 28 51 ± 32 5932 ± 367
Control serum/LPS/TVX 4773 ± 423* 1166 ± 182 159 ± 26 666 ± 85* 2770 ± 283*
Anti-PMN serum/LPS/TVX 2318 ± 258*# 107 ± 47*# 71 ± 8*# 157 ± 30# 1959 ± 232*
  • * Significantly different from Control serum/Veh/Veh-treated group; # significantly different from Control serum/LPS/TVX-treated group (p < 0.05).