RAW 264.7 cells were preincubated for 2 h in the absence or presence of graded concentrations of EA. After being incubated for an additional 2 h in the presence of 10 ng/ml LPS, culture supernatants were harvested and assayed for TNF concentration by ELISA. Control cultures were incubated for 4 h in the absence of either EA or LPS. Results are means ± S.E.M. (n = 5 per condition).
LPS
EA Conc.
TNF Conc.
μM
pg / ml
-
0
72 ± 23
+
0
4683 ± 143
+
7.5
4176 ± 132*
+
15
4143 ± 172*
+
30
3676 ± 67†
+
60
3176 ± 84†
+
120
710 ± 47†
Conc., concentration.
↵*P < 0.01 and †P < 0.001 versus the LPS +/ EA 0 μM condition.