Effects of KMUP-3 and forskolin (100 μM) on cAMP and cGMP levels in TSMCs in the absence and presence of SQ 22536 (100 μM), l-NAME (100 μM), or ODQ (10 μM)
Each value represents the mean ± S.E.M. of three independent experiments.
Treatment | |
|---|---|
| cAMP (pmol/mg protein) | |
| Vehicle | 62.60 ± 6.40 |
| SQ 22536 | 74.32 ± 7.56 |
| Forskolin | 119.15 ± 5.11* |
| Forskolin plus SQ 22536 | 94.33 ± 3.42** |
| KMUP-3 | 128.72 ± 4.57* |
| KMUP-3 plus SQ 22536 | 74.51 ± 3.18** |
| Cyclic GMP (pmol/mg protein) | |
| Vehicle | 1.18 ± 0.06 |
| KMUP-3 | 1.98 ± 0.08* |
| l-NAME | 1.12 ± 0.02 |
| KMUP-3 plus l-NAME | 1.28 ± 0.01** |
| ODQ | 1.12 ± 0.03 |
| KMUP-3 plus ODQ | 1.35 ± 0.04** |