TABLE 1
Effect of type 2 diabetes on toxicity of APAP, BB, and CCl4 in male Swiss-Webster mice
Male SW mice were fed either high fat diet or normal diet. On day 60, male SW mice were injected with either STZ (120 mg/kg i.p. in citrate buffer, pH 4.3) or citrate buffer (10 ml/kg i.p.) alone. On day 71, all DB (high fat diet mice treated with STZ) and non-DB groups [normal diet-fed mice treated with either STZ (ND + STZ) or vehicle (ND), high fat diet-fed mice-injected vehicle (HFD)] were injected with single dose of APAP (600 mg/kg i.p. in warm basic saline, pH 8), CCl4 (1.25 ml/kg i.p. in corn oil), or BB (0.5 ml/kg i.p. in corn oil). Survival and mortality were observed three times on the 1st day and twice daily thereafter for a total of 14 days. No mortality was observed in the vehicle-treated DB and non-DB mice. Number of deaths and time were recorded.