Real-time quantitative RT-PCR: TaqMan primers and probes
Forward (F) and reverse primers (R) (5′-3′) for PCR amplification and fluorogenic probes (5′-3′) are given for analysis of MMP-2, MMP-9, TIMP-1, TIMP-2, α-SMA, collagen III, and TGF-β1, respectively.
Gene | Primer Sequence | Probe |
|---|---|---|
| MMP-2 | F: AGGAGACAAGTTCTGGAGATACAATGA | ATCGCAGACTCCTGGAATGCCATCC |
| R: CCACGACAGCATCCAGGTTA | ||
| MMP-9 | F: TGGAACTCACACAACGTCTTTCA | CAAGACAAGGCCTATTTCTGCCATGACAA |
| R: TCACCCGGTTGTGGAAACTC | ||
| TIMP-1 | F: ATCAAGATGACTAAGATGCTCAAAGG | CCACAGGTTTCCGGTTCGCCTACA |
| R: GGCCCGCGATGAGAAACT | ||
| TIMP-2 | F: ACCCAGAAGAAGAGCCTAAACCA | CACACGCTGCCCTATGATCCCATG |
| R: GTCCATCCAGAGGCACTCATC | ||
| α-SMA | F: CTTCCAGCCATCTTTCATTGG | TCGGCGGGCATCCACGAAAC |
| R: ATATCACACTTCATGATGCTGTTATAGGT | ||
| Collagen III | F: TGAAATTCTGCCACCCTGAACT | AAGAGTGGAGAATACTGGGT |
| R: CATCTTGCAGCCTTGGTTAGG | ||
| TGF-β1 | F: CCTGCAAGACCATCGACATG | AAGCGCATCGAAGCCATCCGTG |
|
| R: TGGCGAGCCTTAGTTTGGA |
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