Sequences of the primers specific for rat β1-AR, β2-AR, β3-AR, and β-actin
Primer | PCR Product | Annealing Temperature | No. of Cycles | Reference | ||
|---|---|---|---|---|---|---|
| base pairs | °C | |||||
| β1-AR | Forward | 5′–CTCACCAACCTCTTCATCATG–3′ | ||||
| Reverse | 5′–GAAGCGGCGCTCGCAGCTGTCG–3′ | 522 | 60 | 33 | Podlowski et al. (1998) | |
| β2-AR | Forward | 5′–GAGACCCTGTGCGTGATTGC–3′ | ||||
| Reverse | 5′–CCTGCTCCACCTGGCTGAGG–3′ | 387 | 60 | 33 | Podlowski et al. (1998) | |
| β3-AR | Forward | 5′–CCAACTCTGCCTTCAACCCGCTCA–3′ | ||||
| Reverse | 5′–CCTTCATGTGGGAAATGGACGCTC–3′ | 204 | 60 | 40 | Bensaid et al. (1993) | |
| β-Actin | Forward | 5′–CGTAAAGACCTCTATGCCAA–3′ | ||||
|
| Reverse | 5′–GGTGTAAAACGCAGCTCAGT–3′ | 301 | 55 | 25 |
|